Expression of an engrailed -like gene during development of the early embryonic chick nervous system

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dc.contributor.author Gardner, Charles A. en_US
dc.contributor.author Darnell, D. K. en_US
dc.contributor.author Poole, S. J. en_US
dc.contributor.author Ordahl, C. P. en_US
dc.contributor.author Barald, Kate F. en_US
dc.date.accessioned 2007-04-06T18:40:30Z
dc.date.available 2007-04-06T18:40:30Z
dc.date.issued 1988-10 en_US
dc.identifier.citation Gardner, C. A.; Darnell, D. K.; Poole, S. J.; Ordahl, C. P.; Barald, K. F. (1988)."Expression of an engrailed -like gene during development of the early embryonic chick nervous system." Journal of Neuroscience Research 21(2-4): 426-437. <http://hdl.handle.net/2027.42/50222> en_US
dc.identifier.issn 0360-4012 en_US
dc.identifier.issn 1097-4547 en_US
dc.identifier.uri http://hdl.handle.net/2027.42/50222
dc.identifier.uri http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2464080&dopt=citation en_US
dc.description.abstract The engrailed gene has been identified in Drosophila as an important developmental gene involved in the control of segmentation. Here we describe the embryonic expression of a chicken gene, ChickEn (Darnell et al.: J Cell Biol 103(5):311a, 1986), which contains homology to the Drosophila engrailed gene. Northern blots of early chick embryo tissue poly(A) + RNA resulted in hybridization to at least three bands expressed predominantly in the brain/head region when probed with ChickEn genomic fragments. Eight cDNA clones generated from embryonic day 6 (stage 29–30) chick brain poly(A) + RNA are identical in their nucleotide sequence with the ChickEn genomic clone. In situ hybridization to sections of 4-day (stage 24) embryos indicated that ChickEn transcripts were concentrated in the posterior mesencephalon and anterior metencephalon. In cultures of chick cranial neural crest cells (eight to nine somites; stage 9) ChickEn transcripts were localized in a subset (approx. 8%) of cells examined after 2 days in culture. A mouse monoclonal antibody, inv-4D9D4, made by Coleman and Kornberg recognizes the engrailed -like homeo domain of the engrailed and invected proteins (Martin-Blanco, Coleman, and Kornberg, personal communication). Patel, Coleman, Kornberg and Goodman (unpublished) have shown that this antibody binds to the hindbrain of 2-day-old chick embryos. We have confirmed these results and shown that this antibody binds to the same region of 4-day (stage 24) chick brains that in situ hybridization showed contained ChickEn transcripts. This antibody also recognizes a homeo domain-containing ChickEn peptide expressed as a beta-galactosidase fusion protein in Drosophila cell culture. We have not detected ChickEn protein in any tissue prior to eight to nine somites (stage 9). These results delineate the major expression pattern of the ChickEn gene during early (prior to stage 30) embryonic development in the chick. en_US
dc.format.extent 1419457 bytes
dc.format.extent 3118 bytes
dc.format.mimetype application/pdf
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dc.publisher Wiley Subscription Services, Inc., A Wiley Company en_US
dc.subject.other Life and Medical Sciences en_US
dc.subject.other Neuroscience, Neurology and Psychiatry en_US
dc.title Expression of an engrailed -like gene during development of the early embryonic chick nervous system en_US
dc.rights.robots IndexNoFollow en_US
dc.subject.hlbsecondlevel Molecular, Cellular and Developmental Biology en_US
dc.subject.hlbsecondlevel Neurosciences en_US
dc.subject.hlbsecondlevel Psychology en_US
dc.subject.hlbsecondlevel Public Health en_US
dc.subject.hlbtoplevel Health Sciences en_US
dc.subject.hlbtoplevel Science en_US
dc.subject.hlbtoplevel Social Sciences en_US
dc.description.peerreviewed Peer Reviewed en_US
dc.contributor.affiliationum Department of Anatomy and Cell Biology, University of Michigan, Ann Arbor, University of California, San Francisco en_US
dc.contributor.affiliationum Department of Anatomy and Cell Biology, University of Michigan, Ann Arbor, University of California, San Francisco ; Department of Anatomy and Cell Biology, University of Michigan, Ann Arbor, MI 48109 en_US
dc.contributor.affiliationother Departments of Anatomy, University of California, San Francisco en_US
dc.contributor.affiliationother Department of Biochemistry and Biophysics, University of California, San Francisco en_US
dc.contributor.affiliationother Departments of Anatomy, University of California, San Francisco en_US
dc.identifier.pmid 2464080 en_US
dc.description.bitstreamurl http://deepblue.lib.umich.edu/bitstream/2027.42/50222/1/490210234_ftp.pdf en_US
dc.identifier.doi http://dx.doi.org/10.1002/jnr.490210234 en_US
dc.identifier.source Journal of Neuroscience Research en_US
dc.owningcollname Interdisciplinary and Peer-Reviewed
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