Interferon-Γ inhibits DNA synthesis and insulin-like growth factor-II expression in human neuroblastoma cells
dc.contributor.author | Martin, Donna M. | en_US |
dc.contributor.author | Carlson, Robert O. | en_US |
dc.contributor.author | Feldman, Eva L. | en_US |
dc.date.accessioned | 2007-04-06T18:40:56Z | |
dc.date.available | 2007-04-06T18:40:56Z | |
dc.date.issued | 1993-04-01 | en_US |
dc.identifier.citation | Martin, D. M.; Carlson, R. O.; Feldman, E. L. (1993)."Interferon-Γ inhibits DNA synthesis and insulin-like growth factor-II expression in human neuroblastoma cells." Journal of Neuroscience Research 34(5): 489-501. <http://hdl.handle.net/2027.42/50226> | en_US |
dc.identifier.issn | 0360-4012 | en_US |
dc.identifier.issn | 1097-4547 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/50226 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8478984&dopt=citation | en_US |
dc.description.abstract | Interferon-Γ (IFN-Γ) is known to be an antiproliferative, differentiation agent in many cell types, including neuroblastoma. In this study, we determined the effects of IFN-Γ on cellular growth and expression of insulin-like growth factor II (IGF-II) and IGF receptors in the human neuroblastoma cell line SH-SY5Y. Incubation of SH-SY5Y cells in IFN-Γ (20–100 U/ml) induced the formation of long neuritic processes. IFN-Γ treatment also induced decreases in [ 3 H]TdR incorporation, as well as serum-dependent changes in cell number. Treatment with IFN-Γ reduced cell number 33% in the presence of serum but had no effect on cell number in the absence of serum. IGF-II mRNA content was 60% inhibited by IFN-Γ, and was not serum dependent. The concentration of immunoreactive IGF-II in SH-SY5Y conditioned medium was also reduced in the presence of IFN-Γ, to less than half of control levels. In contrast, type I IGF receptor mRNA content was increased more than three-fold after treatment with IFN-Γ and serum. Co-incubation in IFN-Γ (20–100 U/ml) and IGF-II on (3–10 nM) prevented the inhibitory effects of IFN-Γ on [ 3 H]TdR ncorporation in serum-free media. Our results suggest that IFN-Γ may inhibit DNA synthesis and cell growth by interfering with an IGF-II/type I IGF receptor autocrine growth or survival mechanism. | en_US |
dc.format.extent | 2088770 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Neuroscience, Neurology and Psychiatry | en_US |
dc.title | Interferon-Γ inhibits DNA synthesis and insulin-like growth factor-II expression in human neuroblastoma cells | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbsecondlevel | Psychology | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Social Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Neurology and the Neuroscience Program, The University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Biochemistry, The University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Neurology and the Neuroscience Program, The University of Michigan, Ann Arbor, Michigan ; 1120C Neuroscience Laboratory, 1103 E. Huron Ave., Ann Arbor, MI 48104 | en_US |
dc.identifier.pmid | 8478984 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/50226/1/490340502_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/jnr.490340502 | en_US |
dc.identifier.source | Journal of Neuroscience Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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