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Brain microvessel endothelial cells in tissue culture: A model for study of blood-brain barrier permeability

dc.contributor.authorBowman, Phillip D.en_US
dc.contributor.authorEnnis, Steven R.en_US
dc.contributor.authorRarey, Kyle E.en_US
dc.contributor.authorLorris Betz, A.en_US
dc.contributor.authorGoldstein, Gary W.en_US
dc.date.accessioned2007-04-06T18:49:44Z
dc.date.available2007-04-06T18:49:44Z
dc.date.issued1983-10en_US
dc.identifier.citationBowman, Phillip D.; Ennis, Steven R.; Rarey, Kyle E.; Lorris Betz, A.; Goldstein, Gary W. (1983)."Brain microvessel endothelial cells in tissue culture: A model for study of blood-brain barrier permeability." Annals of Neurology 14(4): 396-402. <http://hdl.handle.net/2027.42/50304>en_US
dc.identifier.issn0364-5134en_US
dc.identifier.issn1531-8249en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/50304
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6638956&dopt=citationen_US
dc.description.abstractEndothelial cells were prepared from bovine brain microvessels and grown in tissue culture. They contained factor VIII/von Willebrand antigen, the most specific marker available for determination of the endothelial origin of cells in culture. The cultured cells formed complex tight junctions and contained few pinocytotic vessels. These properties are responsible for formation of the blood-brain barrier in vivo. When monolayers of the endothelial cells were exposed briefly to a calcium-free solution or treated with 1.6 M arabinose, distinctive morphological changes occurred in the intercellular contacts. In either case, a normal structure was reestablished following return to control medium. To assess the effect of these treatments on transcellular permeability, we measured the movement of sucrose labeled with carbon 14 across a monolayer of endothelial cells cultured on a collagen-coated nylon mesh. Removal of external calcium increased the rate of sucrose movement by 120%; the arabinose treatment increased transcellular flux by 40%.en_US
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dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
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dc.publisherLittle, Brown and Companyen_US
dc.publisherWiley Periodiocals, Inc.en_US
dc.subject.otherLife and Medical Sciencesen_US
dc.subject.otherNeuroscience, Neurology, and Psychiatryen_US
dc.titleBrain microvessel endothelial cells in tissue culture: A model for study of blood-brain barrier permeabilityen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelPsychiatryen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartments of Pediatrics and Neurology University of Michigan, Ann Arbor, MI 48109 ; Department of Pediatrics, R6060 Kresge II, Ann Arbor, MI 48109en_US
dc.contributor.affiliationumDepartments of Pediatrics and Neurology University of Michigan, Ann Arbor, MI 48109en_US
dc.contributor.affiliationumOtorhinolaryngology, University of Michigan, Ann Arbor, MI 48109en_US
dc.contributor.affiliationumDepartments of Pediatrics and Neurology University of Michigan, Ann Arbor, MI 48109en_US
dc.contributor.affiliationumDepartments of Pediatrics and Neurology University of Michigan, Ann Arbor, MI 48109en_US
dc.identifier.pmid6638956en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/50304/1/410140403_ftp.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1002/ana.410140403en_US
dc.identifier.sourceAnnals of Neurologyen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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