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dc.contributor.authorSilverstein, Faye Sarahen_US
dc.contributor.authorSimpson, Jenniferen_US
dc.contributor.authorGordon, Kevin E.en_US
dc.date.accessioned2007-04-06T18:53:05Z
dc.date.available2007-04-06T18:53:05Z
dc.date.issued1990-10en_US
dc.identifier.citationSilverstein, Faye S.; Simpson, Jennifer; Gordon, Kevin E. (1990)."Hypoglycemia alters striatal amino acid efflux in perinatal rats: An in vivo microdialysis study." Annals of Neurology 28(4): 516-521. <http://hdl.handle.net/2027.42/50336>en_US
dc.identifier.issn0364-5134en_US
dc.identifier.issn1531-8249en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/50336
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1979220&dopt=citationen_US
dc.description.abstractIn adult brain, during insulin-induced hypoglycemia, striatal extracellular fluid concentrations of the excitatory amino acids glutamate and aspartate rise markedly (fourfold to tenfold). In this study, we used in vivo microdialysis to determine if insulin-induced hypoglycemia altered striatal amino acid efflux in similar fashion in the immature brain. Microdialysis probes were inserted into the right striatum of rats on postnatal day 7. After a 2-hour recovery period, in each animal a 30-minute baseline sample was obtained. Then insulin (0.6 Μ/Kg, intraperitoneal injection) was administered (n = 6) and dialysate sampling was continued over the next 210 minutes (terminal blood glucose level < 5 mg/dl). Untreated control rats (n = 6) were sampled over the same time interval. After pre-column derivatization with o -Phthaldialdehyde, dialysate samples were assayed by high-pressure liquid chromatography with electrochemical detection to measure their amino acid content; eight amino acids (glutamate, aspartate, taurine, glutamine, alanine, serine glycine, and asparagine) were consistently detected. In controls, amino acid efflux did not change over 4 hours. In hypoglycemic animals, glutamate efflux increased (peak: 238 ± 85% of baseline, p = 0.02, repeated measures analysis of variance [ANOVA]), glutamine efflux declined (to 44 ± 5% of baseline, P = 0.002, ANOVA), and taurine efflux increased (up to 310 ± 120% of baseline; p < 0.06, ANOVA). In contrast with 9-to 12-fold increases in aspartate efflux reported in adult striatum, asparate efflux increased only slighty (to 174 ± 69% of baseline; not significant). In immature rodent brain, overall trends in striatal amino acid efflux during hypoglycemia are similar to patterns reported in adult animals; however, there are major differences in the timing, duration, and magnitude of these responses.en_US
dc.format.extent644723 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.publisherWiley Subscription Services, Inc., A Wiley Companyen_US
dc.subject.otherLife and Medical Sciencesen_US
dc.subject.otherNeuroscience, Neurology, and Psychiatryen_US
dc.titleHypoglycemia alters striatal amino acid efflux in perinatal rats: An in vivo microdialysis studyen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelPsychiatryen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartments of Pediatrics and Neurology, University of Michigan, Ann Arbor, MI ; Room 6028, Kresge II, Box 0570, University of Michigan, Ann Arbor, MI 48109en_US
dc.contributor.affiliationumDepartments of Pediatrics and Neurology, University of Michigan, Ann Arbor, MIen_US
dc.contributor.affiliationumDepartments of Pediatrics and Neurology, University of Michigan, Ann Arbor, MIen_US
dc.identifier.pmid1979220en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/50336/1/410280408_ftp.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1002/ana.410280408en_US
dc.identifier.sourceAnnals of Neurologyen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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