Selective culture of mitotically active human Schwann cells from adult sural nerves
dc.contributor.author | Rutkowski, J. Lynn | en_US |
dc.contributor.author | Tennekoon, Gihan I. | en_US |
dc.contributor.author | McGillicuddy, John E. | en_US |
dc.date.accessioned | 2007-04-06T18:54:41Z | |
dc.date.available | 2007-04-06T18:54:41Z | |
dc.date.issued | 1992-06 | en_US |
dc.identifier.citation | Rutkowski, J. Lynn; Tennekoon, Gihan I.; McGillicuddy, John E. (1992)."Selective culture of mitotically active human Schwann cells from adult sural nerves." Annals of Neurology 31(6): 580-586. <http://hdl.handle.net/2027.42/50351> | en_US |
dc.identifier.issn | 0364-5134 | en_US |
dc.identifier.issn | 1531-8249 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/50351 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1514771&dopt=citation | en_US |
dc.description.abstract | We devised a simple method to isolate mitotically active human Schwann cells from sural nerve biopsy specimens and expand the population in culture. Nerve fascicles were treated with cholera toxin for 7 days in culture before dissociation, which increased the cell yield at least twenty-five–fold over immediated tissue dissociation. Digesting the tissue completely with enzymes in serum-containing medium resulted in the highest cell viability, and released 2 to 6 × 10 4 cells/mg of tissue. Seeding the cells on a poly- L -lysine substrate in a small volume of serum-free medium optimized the plating efficiency. Although Schwann cells comprised 90% of the initial culture population, their numbers declined over time due to a faster mitotic rate of the fibroblasts in the presence of cholera toxin alone. However, treating the cultures with a combination of cholera toxin and forskolin, which act synergistically to elevate cyclic AMP levels, inhibited fibroblast growth without causing Schwann cell toxicity. Adding glial growth factor to the adenyl cyclase activators maximized Schwann cell proliferation, and the population rapidly and selectively expanded. Therefore, it should be possible to generate large numbers of Schwann cells from diseased nerves to study defects in cell function or from normal nerves to study the effects of Schwann cell grafts on neuronal regeneration. | en_US |
dc.format.extent | 1684041 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Neuroscience, Neurology, and Psychiatry | en_US |
dc.title | Selective culture of mitotically active human Schwann cells from adult sural nerves | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Psychiatry | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Departments of Pediatrics, University of Michigan, Ann Arbor, MI ; University of Michigan, Division of Pediatric Neurology, Room 6060, Kresge II, Ann Arbor, MI 48109-0570 | en_US |
dc.contributor.affiliationum | Departments of Neurology, University of Michigan, Ann Arbor, MI | en_US |
dc.contributor.affiliationum | Departments of Neurosurgery, University of Michigan, Ann Arbor, MI | en_US |
dc.identifier.pmid | 1514771 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/50351/1/410310603_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/ana.410310603 | en_US |
dc.identifier.source | Annals of Neurology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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