Selective culture of mitotically active human Schwann cells from adult sural nerves

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dc.contributor.author Rutkowski, J. Lynn en_US
dc.contributor.author Tennekoon, Gihan I. en_US
dc.contributor.author McGillicuddy, John E. en_US
dc.date.accessioned 2007-04-06T18:54:41Z
dc.date.available 2007-04-06T18:54:41Z
dc.date.issued 1992-06 en_US
dc.identifier.citation Rutkowski, J. Lynn; Tennekoon, Gihan I.; McGillicuddy, John E. (1992)."Selective culture of mitotically active human Schwann cells from adult sural nerves." Annals of Neurology 31(6): 580-586. <http://hdl.handle.net/2027.42/50351> en_US
dc.identifier.issn 0364-5134 en_US
dc.identifier.issn 1531-8249 en_US
dc.identifier.uri http://hdl.handle.net/2027.42/50351
dc.identifier.uri http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1514771&dopt=citation en_US
dc.description.abstract We devised a simple method to isolate mitotically active human Schwann cells from sural nerve biopsy specimens and expand the population in culture. Nerve fascicles were treated with cholera toxin for 7 days in culture before dissociation, which increased the cell yield at least twenty-five–fold over immediated tissue dissociation. Digesting the tissue completely with enzymes in serum-containing medium resulted in the highest cell viability, and released 2 to 6 × 10 4 cells/mg of tissue. Seeding the cells on a poly- L -lysine substrate in a small volume of serum-free medium optimized the plating efficiency. Although Schwann cells comprised 90% of the initial culture population, their numbers declined over time due to a faster mitotic rate of the fibroblasts in the presence of cholera toxin alone. However, treating the cultures with a combination of cholera toxin and forskolin, which act synergistically to elevate cyclic AMP levels, inhibited fibroblast growth without causing Schwann cell toxicity. Adding glial growth factor to the adenyl cyclase activators maximized Schwann cell proliferation, and the population rapidly and selectively expanded. Therefore, it should be possible to generate large numbers of Schwann cells from diseased nerves to study defects in cell function or from normal nerves to study the effects of Schwann cell grafts on neuronal regeneration. en_US
dc.format.extent 1684041 bytes
dc.format.extent 3118 bytes
dc.format.mimetype application/pdf
dc.format.mimetype text/plain
dc.publisher Wiley Subscription Services, Inc., A Wiley Company en_US
dc.subject.other Life and Medical Sciences en_US
dc.subject.other Neuroscience, Neurology, and Psychiatry en_US
dc.title Selective culture of mitotically active human Schwann cells from adult sural nerves en_US
dc.type Article en_US
dc.rights.robots IndexNoFollow en_US
dc.subject.hlbsecondlevel Psychiatry en_US
dc.subject.hlbtoplevel Health Sciences en_US
dc.description.peerreviewed Peer Reviewed en_US
dc.contributor.affiliationum Departments of Pediatrics, University of Michigan, Ann Arbor, MI ; University of Michigan, Division of Pediatric Neurology, Room 6060, Kresge II, Ann Arbor, MI 48109-0570 en_US
dc.contributor.affiliationum Departments of Neurology, University of Michigan, Ann Arbor, MI en_US
dc.contributor.affiliationum Departments of Neurosurgery, University of Michigan, Ann Arbor, MI en_US
dc.identifier.pmid 1514771 en_US
dc.description.bitstreamurl http://deepblue.lib.umich.edu/bitstream/2027.42/50351/1/410310603_ftp.pdf en_US
dc.identifier.doi http://dx.doi.org/10.1002/ana.410310603 en_US
dc.identifier.source Annals of Neurology en_US
dc.owningcollname Interdisciplinary and Peer-Reviewed
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