The preparation of ultrathin frozen sections for immunocytochemistry at the electron microscope level
Christensen, A. Kent; Komorowski, Thomas E.
1985
Citation
Christensen, A. Kent; Komorowski, Thomas E. (1985)."The preparation of ultrathin frozen sections for immunocytochemistry at the electron microscope level." Journal of Electron Microscopy Technique 2(5): 497-507. <http://hdl.handle.net/2027.42/50384>
Abstract
A description of ultrathin frozen sectioning is presented. Small pieces of lightly-fixed but unembedded tissue are cryoprotected in 80% sucrose, mounted on chucks and frozen in liquid nitrogen. Frozen sections approximately 100 nm thick for electron microscopy are cut at about −90°C, using a cryosectioning unit mounted on a conventional ultramicrotome. Sections are picked up from the surface of the knife with a droplet of 80% sucrose, and are applied to membrane-coated EM grids at room temperature. The mounted sections are then used for EM immunocytochemistry. Essentially the same sectioning procedure can provide frozen sections 1 Μm thick for light microscope immunocytochemistry.Publisher
Wiley Subscription Services, Inc., A Wiley Company
ISSN
0741-0581 1553-0817
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