Calmodulin is associated with microtubules forming in PTK 1 cells upon release from nocodazole treatment

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dc.contributor.author Sweet, Stuart C. en_US
dc.contributor.author Rogers, Charles M. en_US
dc.contributor.author Welsh, Michael J. en_US
dc.date.accessioned 2007-04-06T19:00:03Z
dc.date.available 2007-04-06T19:00:03Z
dc.date.issued 1989 en_US
dc.identifier.citation Sweet, Stuart C.; Rogers, Charles M.; Welsh, Michael J. (1989)."Calmodulin is associated with microtubules forming in PTK 1 cells upon release from nocodazole treatment." Cell Motility and the Cytoskeleton 12(2): 113-122. <http://hdl.handle.net/2027.42/50401> en_US
dc.identifier.issn 0886-1544 en_US
dc.identifier.issn 1097-0169 en_US
dc.identifier.uri http://hdl.handle.net/2027.42/50401
dc.identifier.uri http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2713899&dopt=citation en_US
dc.description.abstract To investigate the association of calmodulin (CaM) with microtubules (MTs) in the mitotic apparatus (MA), the distributions of CaM and tubulin were examined in cells in which the normal spindle organization had been altered. A fluorescent CaM conjugate with tetramethylrhodamine isothiocyanate (CaM-TRITC) and a dichlorotriazinyl aminofluorescein conjugate with tubulin (tubulin-DTAF) were injected into cells that had been treated with the MT inhibitor nocodazole. With moderate nocodazole concentration (0.3 Μg/ml, 37°C, 4 h) in live cells, CaM-TRITC and tubulin-DTAF concentrated identically on or near the centrosomes and kinetochores. In serial sections of these cells, small MT segments were observed by transmission electron microscopy (TEM) in the regions where fluorescent protein had concentrated. When a higher drug concentration was used (3.0 Μg/ml, 37°C, 4 h), no regions of CaM-TRITC or tubulin-DTAF localization were observed, and no MTs were observed when serial sections were examined by TEM. However, following release from the high-concentration nocodazole block, CaM-TRITC colocalized with newly formed MTs at the kinetochores and centrosomes. Later in the recovery period, when chromosome-to-pole fibers had formed, CaM association with kinetochores diminished, ultimately attaining its normal pole-proximal association with kinetochore MTs in cells that progressed through mitosis. We interpret these observations as supporting the hypothesis that in the MA, CaM attains a physical association with kinetochore MTs and suggest that CaM-associated MTs may be inherently more stable. en_US
dc.format.extent 1174392 bytes
dc.format.extent 3118 bytes
dc.format.mimetype application/pdf
dc.format.mimetype text/plain
dc.publisher Wiley Subscription Services, Inc., A Wiley Company en_US
dc.subject.other Life and Medical Sciences en_US
dc.subject.other Cell & Developmental Biology en_US
dc.title Calmodulin is associated with microtubules forming in PTK 1 cells upon release from nocodazole treatment en_US
dc.type Article en_US
dc.rights.robots IndexNoFollow en_US
dc.subject.hlbsecondlevel Molecular, Cellular and Developmental Biology en_US
dc.subject.hlbtoplevel Health Sciences en_US
dc.subject.hlbtoplevel Science en_US
dc.description.peerreviewed Peer Reviewed en_US
dc.contributor.affiliationum Department of Anatomy and Cell Biology, University of Michigan Medical School, Ann Arbor en_US
dc.contributor.affiliationum Department of Anatomy and Cell Biology, University of Michigan Medical School, Ann Arbor en_US
dc.contributor.affiliationum Department of Anatomy and Cell Biology, University of Michigan Medical School, Ann Arbor ; 4728 Medical Science II, Department of Anatomy and Cell Biology, University of Michigan Medical School, Ann Arbor, MI 48109-0616 en_US
dc.identifier.pmid 2713899 en_US
dc.description.bitstreamurl http://deepblue.lib.umich.edu/bitstream/2027.42/50401/1/970120206_ftp.pdf en_US
dc.identifier.doi http://dx.doi.org/10.1002/cm.970120206 en_US
dc.identifier.source Cell Motility and the Cytoskeleton en_US
dc.owningcollname Interdisciplinary and Peer-Reviewed
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