Ectopic expression of chloramphenicol acetyltransferase (CAT) in the cerebellum in mice transgenic for a carbonic anhydrase II promoter-CAT construct that is without apparent phenotypic effect
dc.contributor.author | Erickson, Robert P. | en_US |
dc.contributor.author | Bevilacqua, Arturo | en_US |
dc.contributor.author | Venta, Patrick J. | en_US |
dc.contributor.author | Karolyi, Jill | en_US |
dc.contributor.author | Tashian, Richard E. | en_US |
dc.date.accessioned | 2007-04-06T19:01:39Z | |
dc.date.available | 2007-04-06T19:01:39Z | |
dc.date.issued | 1990-10 | en_US |
dc.identifier.citation | Erickson, Robert P.; Bevilacqua, Arturo; Venta, Patrick J.; Karolyi, Jill; Tashian, Richard E. (1990)."Ectopic expression of chloramphenicol acetyltransferase (CAT) in the cerebellum in mice transgenic for a carbonic anhydrase II promoter-CAT construct that is without apparent phenotypic effect." Molecular Reproduction and Development 27(2): 102-109. <http://hdl.handle.net/2027.42/50416> | en_US |
dc.identifier.issn | 1040-452X | en_US |
dc.identifier.issn | 1098-2795 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/50416 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2123391&dopt=citation | en_US |
dc.description.abstract | We have developed six transgenic lines of mice with constructs containing presumptive 5′ regulatory regions of carbonic anhydrase II (CA II). Four of the lines contained 1,100 bases of the 5′ flanking region of the human CA II gene, and two transgenic lines resulted from a construct containing 500 bases of the 5′ flanking region of the mouse CA II gene. Tissue-specific expression of the chloramphenicol acetyltransferase (CAT) gene was not obtained in any of the transgenic lines. One of the transgenic lines was found to have high levels of expression of CAT in cerebellum. This expression persisted through multiple generations and was independent of the parental origin of the transgene. On the assumption that the expression was due to the insertion of the transgene in or near a gene expressed normally in cerebellum, homozygous mice were bred for the transgenic insert to see if a mutation might have been induced. Homozygous mice were found and seemed to be normal in all aspects of their phenotype studied. Thus, in this case, neither the insertion of the gene nor the ectopic expression of CAT seemed to be harmful to the animals. | en_US |
dc.format.extent | 795514 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Cell & Developmental Biology | en_US |
dc.title | Ectopic expression of chloramphenicol acetyltransferase (CAT) in the cerebellum in mice transgenic for a carbonic anhydrase II promoter-CAT construct that is without apparent phenotypic effect | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Obstetrics and Gynecology | en_US |
dc.subject.hlbsecondlevel | Women's and Gender Studies | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Kinesiology and Sports | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Humanities | en_US |
dc.subject.hlbtoplevel | Social Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Human Genetics, University of Michigan Medical School, Ann Arbor, Michigan ; Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, Michigan ; University of Michigan Medical School, Department of Human Genetics, 3704 Medical Science II, Ann Arbor, MI 48109-0618 | en_US |
dc.contributor.affiliationum | Department of Human Genetics, University of Michigan Medical School, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Human Genetics, University of Michigan Medical School, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Human Genetics, University of Michigan Medical School, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Human Genetics, University of Michigan Medical School, Ann Arbor, Michigan | en_US |
dc.identifier.pmid | 2123391 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/50416/1/1080270204_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/mrd.1080270204 | en_US |
dc.identifier.source | Molecular Reproduction and Development | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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