Changes in the rat heart proteome induced by exercise training: Increased abundance of heat shock protein hsp20
dc.contributor.author | Boluyt, Marvin O. | en_US |
dc.contributor.author | Brevick, Julie L. | en_US |
dc.contributor.author | Rogers, David S. | en_US |
dc.contributor.author | Randall, Michael J. | en_US |
dc.contributor.author | Scalia, Antony F. | en_US |
dc.contributor.author | Li, Zhao Bo | en_US |
dc.date.accessioned | 2007-05-02T14:19:54Z | |
dc.date.available | 2007-05-02T14:19:54Z | |
dc.date.issued | 2006-05 | en_US |
dc.identifier.citation | Boluyt, Marvin O.; Brevick, Julie L.; Rogers, David S.; Randall, Michael J.; Scalia, Antony F.; Li, Zhao Bo (2006). "Changes in the rat heart proteome induced by exercise training: Increased abundance of heat shock protein hsp20." PROTEOMICS 6(10): 3154-3169. <http://hdl.handle.net/2027.42/50684> | en_US |
dc.identifier.issn | 1615-9853 | en_US |
dc.identifier.issn | 1615-9861 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/50684 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=16586429&dopt=citation | en_US |
dc.description.abstract | Chronic exercise training elicits adaptations in the heart that improve pump function and confer cardioprotection. To identify molecular mechanisms by which exercise training stimulates this favorable phenotype, a proteomic approach was employed to detect rat cardiac proteins that were differentially expressed or modified after exercise training. Exercise-trained rats underwent six weeks of progressive treadmill training five days/week, 0% 14grade, using an interval training protocol. Sedentary control rats were age- and weight-matched to the exercise-trained rats. Hearts were harvested at various times (0–72 14h) after the last bout of exercise and were used to generate 2-D electrophoretic proteome maps and immunoblots. Compared with hearts of sedentary rats, 26 14protein spot intensities were significantly altered in hypertrophied hearts of exercise-trained rats ( p 14<0.05), and 12 14spots appeared exclusively on gels from hearts of exercise-trained rats. Immunoblotting confirmed that chronic exercise training, but not a single bout of exercise, elicited a ˜2.5-fold increase in the abundance of one of the candidate proteins in the heart, a ˜20 14kDa heat shock protein (hsp20) that persisted for at least 72 14h of detraining. Thus, exercise training alters the cardiac proteome of the rat heart; the changes include a marked increase in the expression of hsp20. | en_US |
dc.format.extent | 674963 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | WILEY-VCH Verlag | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.title | Changes in the rat heart proteome induced by exercise training: Increased abundance of heat shock protein hsp20 | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Laboratory of Molecular Kinesiology, Center for Exercise Research, Division of Kinesiology, The University of Michigan, Ann Arbor, MI, USA ; The University of Michigan, Laboratory of Molecular Kinesiology, 401 Washtenaw Avenue, Ann Arbor, MI 48109-2214, USA Fax: +1-734-936-1925 | en_US |
dc.contributor.affiliationum | Laboratory of Molecular Kinesiology, Center for Exercise Research, Division of Kinesiology, The University of Michigan, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Laboratory of Molecular Kinesiology, Center for Exercise Research, Division of Kinesiology, The University of Michigan, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Laboratory of Molecular Kinesiology, Center for Exercise Research, Division of Kinesiology, The University of Michigan, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Laboratory of Molecular Kinesiology, Center for Exercise Research, Division of Kinesiology, The University of Michigan, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Laboratory of Molecular Kinesiology, Center for Exercise Research, Division of Kinesiology, The University of Michigan, Ann Arbor, MI, USA | en_US |
dc.identifier.pmid | 16586429 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/50684/1/3154_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/pmic.200401356 | en_US |
dc.identifier.source | PROTEOMICS | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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