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Myeloperoxidase accumulates at the neutrophil surface and enhances cell metabolism and oxidant release during pregnancy

dc.contributor.authorKindzelskii, Andrei L.en_US
dc.contributor.authorClark, Andrea J.en_US
dc.contributor.authorEspinoza, Jimmyen_US
dc.contributor.authorMaeda, Nobuyoen_US
dc.contributor.authorAratani, Yasuakien_US
dc.contributor.authorRomero, Robertoen_US
dc.date.accessioned2007-07-11T18:14:07Z
dc.date.available2007-07-11T18:14:07Z
dc.date.issued2006-06en_US
dc.identifier.citationKindzelskii, Andrei L.; Clark, Andrea J.; Espinoza, Jimmy; Maeda, Nobuyo; Aratani, Yasuaki; Romero, Roberto (2006). "Myeloperoxidase accumulates at the neutrophil surface and enhances cell metabolism and oxidant release during pregnancy." European Journal of Immunology 36(6): 1619-1628. <http://hdl.handle.net/2027.42/55223>en_US
dc.identifier.issn0014-2980en_US
dc.identifier.issn1521-4141en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/55223
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=16688678&dopt=citationen_US
dc.description.abstractPregnancy is a unique immunological state. Pregnancy neutrophils differ from those of non-pregnant women as they cannot be fully activated for oxidant production, but yet have higher levels of unstimulated oxidant production. Although reduced activation is due to decreased hexose monophosphate shunt activity, the mechanism enhancing basal oxidant levels is unknown. We hypothesize that myeloperoxidase (MPO) trafficking affects the basal oxidant release by maternal neutrophils. Immunofluorescence microscopy has demonstrated MPO at the surface of pregnancy neutrophils, whereas non-pregnancy cells do not exhibit surface MPO. Adherent pregnancy neutrophils were characterized by high-amplitude metabolic oscillations, which were blocked by MPO inactivation. Conversely, metabolic oscillatory amplitudes of control neutrophils were heightened by incubation with PMA or exogenous MPO. Importantly, MPO decoration of cell surfaces and high-amplitude metabolic oscillations were observed for neutrophils from pregnant but not from non-pregnant mice. However, cells from pregnant MPO knockout mice did not exhibit MPO expression or high-amplitude metabolic oscillations. Unstimulated neutrophils from pregnant women were found to release reactive oxygen metabolites (ROM) and reactive nitrogen intermediates (RNI), but cells from non-pregnant women did not. MPO inhibition returned ROM and RNI formation to non-pregnant levels. Hence, MPO trafficking influences metabolic activity and oxidant production in pregnancy.en_US
dc.format.extent306413 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.publisherWILEY-VCH Verlagen_US
dc.subject.otherLife and Medical Sciencesen_US
dc.subject.otherMicrobiology and Immunologyen_US
dc.titleMyeloperoxidase accumulates at the neutrophil surface and enhances cell metabolism and oxidant release during pregnancyen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbsecondlevelPublic Healthen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Ophthalmology and Visual Sciences, The University of Michigan Medical School, Ann Arbor, USAen_US
dc.contributor.affiliationumDepartment of Ophthalmology and Visual Sciences, The University of Michigan Medical School, Ann Arbor, USAen_US
dc.contributor.affiliationotherPerinatology Research Branch, National Institute of Child Health and Human Development, Hutzel Hospital, Detroit, USAen_US
dc.contributor.affiliationotherDepartment of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, USAen_US
dc.contributor.affiliationotherKihara Institute for Biological Research, Yokohama City University, Yokohama, Japanen_US
dc.contributor.affiliationotherPerinatology Research Branch, National Institute of Child Health and Human Development, Hutzel Hospital, Detroit, USAen_US
dc.identifier.pmid16688678en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/55223/1/1619_ftp.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1002/eji.200535391en_US
dc.identifier.sourceEuropean Journal of Immunologyen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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