Proteomic analysis of estrogen response of premalignant human breast cells using a 2-D liquid separation/mass mapping technique

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dc.contributor.author Zhao, Jia en_US
dc.contributor.author Zhu, Kan en_US
dc.contributor.author Lubman, David M. en_US
dc.contributor.author Miller, Fred R. en_US
dc.contributor.author Shekhar, Malthy P. V. en_US
dc.contributor.author Gerard, Brigitte en_US
dc.contributor.author Barder, Timothy J. en_US
dc.date.accessioned 2007-07-11T18:18:22Z
dc.date.available 2007-07-11T18:18:22Z
dc.date.issued 2006-07 en_US
dc.identifier.citation Zhao, Jia; Zhu, Kan; Lubman, David M.; Miller, Fred R.; Shekhar, Malthy P. V.; Gerard, Brigitte; Barder, Timothy J. (2006). "Proteomic analysis of estrogen response of premalignant human breast cells using a 2-D liquid separation/mass mapping technique." PROTEOMICS 6(13): 3847-3861. <http://hdl.handle.net/2027.42/55250> en_US
dc.identifier.issn 1615-9853 en_US
dc.identifier.issn 1615-9861 en_US
dc.identifier.uri http://hdl.handle.net/2027.42/55250
dc.identifier.uri http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=16767785&dopt=citation en_US
dc.description.abstract A 2-D liquid-phase separation method based on chromatofocusing and nonporous silica RP-HPLC followed by ESI-TOF-MS was used to analyze proteins in whole cell lysates from estrogen-treated and untreated premalignant, estrogen-responsive cell line MCF10AT1 cells. 2-D mass maps in the pH range 144.6–6.0 were generated with good correlation to theoretical M r values for intact proteins. Proteins were identified based on intact M r , p I and PMF, or MS/MS sequencing. About 300 14unique proteins were identified and 120 14proteins in mass range 5–75 14kDa were quantified upon treatment of estrogen. Around 40 14proteins were found to be more highly expressed (>four-fold) and 17 14were down-regulated (>four-fold) in treated cells. In our study, we found that many altered proteins have characteristics consistent with the development of a malignant phenotype. Some of them have a role in the ras pathway or play an important role in signal pathways. These changed proteins might be essential in the estrogen regulation mechanism. Our study highlights the use of the MCF10AT1 cell line to examine estrogen-induced changes in premalignant breast cells and the ability of the 2-D mass mapping technique to quantitatively study protein expression changes on a proteomic scale. en_US
dc.format.extent 681667 bytes
dc.format.extent 3118 bytes
dc.format.mimetype application/pdf
dc.format.mimetype text/plain
dc.publisher WILEY-VCH Verlag en_US
dc.subject.other Life and Medical Sciences en_US
dc.title Proteomic analysis of estrogen response of premalignant human breast cells using a 2-D liquid separation/mass mapping technique en_US
dc.type Article en_US
dc.rights.robots IndexNoFollow en_US
dc.subject.hlbsecondlevel Biological Chemistry en_US
dc.subject.hlbsecondlevel Chemical Engineering en_US
dc.subject.hlbsecondlevel Chemistry en_US
dc.subject.hlbsecondlevel Materials Science and Engineering en_US
dc.subject.hlbsecondlevel Molecular, Cellular and Developmental Biology en_US
dc.subject.hlbtoplevel Health Sciences en_US
dc.subject.hlbtoplevel Science en_US
dc.subject.hlbtoplevel Engineering en_US
dc.description.peerreviewed Peer Reviewed en_US
dc.contributor.affiliationum Department of Chemistry, University of Michigan, Ann Arbor, MI, USA en_US
dc.contributor.affiliationum Department of Chemistry, University of Michigan, Ann Arbor, MI, USA en_US
dc.contributor.affiliationum Department of Chemistry, University of Michigan, Ann Arbor, MI, USA ; Department of Surgery, University of Michigan Medical Center, Ann Arbor, MI, USA ; Comprehensive Cancer Center, University of Michigan Medical Center, Ann Arbor, MI, USA ; Department of Surgery, The University of Michigan Medical Center, MSRB1, 1150 West Medical Center Drive, Ann Arbor, MI 48109-0656, USA Fax: +1-734-615-8108 en_US
dc.contributor.affiliationother Breast Cancer Program, Barbara Ann Karmanos Cancer Institute, Detroit, MI, USA ; Department of Pathology, Wayne State University School of Medicine, Detroit, MI, USA en_US
dc.contributor.affiliationother Breast Cancer Program, Barbara Ann Karmanos Cancer Institute, Detroit, MI, USA ; Department of Pathology, Wayne State University School of Medicine, Detroit, MI, USA en_US
dc.contributor.affiliationother Breast Cancer Program, Barbara Ann Karmanos Cancer Institute, Detroit, MI, USA en_US
dc.contributor.affiliationother Eprogen, Darien, IL, USA en_US
dc.identifier.pmid 16767785 en_US
dc.description.bitstreamurl http://deepblue.lib.umich.edu/bitstream/2027.42/55250/1/3847_ftp.pdf en_US
dc.identifier.doi http://dx.doi.org/10.1002/pmic.200500195 en_US
dc.identifier.source PROTEOMICS en_US
dc.owningcollname Interdisciplinary and Peer-Reviewed
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