Proteomic analysis of estrogen response of premalignant human breast cells using a 2-D liquid separation/mass mapping technique
dc.contributor.author | Zhao, Jia | en_US |
dc.contributor.author | Zhu, Kan | en_US |
dc.contributor.author | Lubman, David M. | en_US |
dc.contributor.author | Miller, Fred R. | en_US |
dc.contributor.author | Shekhar, Malthy P. V. | en_US |
dc.contributor.author | Gerard, Brigitte | en_US |
dc.contributor.author | Barder, Timothy J. | en_US |
dc.date.accessioned | 2007-07-11T18:18:22Z | |
dc.date.available | 2007-07-11T18:18:22Z | |
dc.date.issued | 2006-07 | en_US |
dc.identifier.citation | Zhao, Jia; Zhu, Kan; Lubman, David M.; Miller, Fred R.; Shekhar, Malthy P. V.; Gerard, Brigitte; Barder, Timothy J. (2006). "Proteomic analysis of estrogen response of premalignant human breast cells using a 2-D liquid separation/mass mapping technique." PROTEOMICS 6(13): 3847-3861. <http://hdl.handle.net/2027.42/55250> | en_US |
dc.identifier.issn | 1615-9853 | en_US |
dc.identifier.issn | 1615-9861 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/55250 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=16767785&dopt=citation | en_US |
dc.description.abstract | A 2-D liquid-phase separation method based on chromatofocusing and nonporous silica RP-HPLC followed by ESI-TOF-MS was used to analyze proteins in whole cell lysates from estrogen-treated and untreated premalignant, estrogen-responsive cell line MCF10AT1 cells. 2-D mass maps in the pH range 144.6–6.0 were generated with good correlation to theoretical M r values for intact proteins. Proteins were identified based on intact M r , p I and PMF, or MS/MS sequencing. About 300 14unique proteins were identified and 120 14proteins in mass range 5–75 14kDa were quantified upon treatment of estrogen. Around 40 14proteins were found to be more highly expressed (>four-fold) and 17 14were down-regulated (>four-fold) in treated cells. In our study, we found that many altered proteins have characteristics consistent with the development of a malignant phenotype. Some of them have a role in the ras pathway or play an important role in signal pathways. These changed proteins might be essential in the estrogen regulation mechanism. Our study highlights the use of the MCF10AT1 cell line to examine estrogen-induced changes in premalignant breast cells and the ability of the 2-D mass mapping technique to quantitatively study protein expression changes on a proteomic scale. | en_US |
dc.format.extent | 681667 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | WILEY-VCH Verlag | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.title | Proteomic analysis of estrogen response of premalignant human breast cells using a 2-D liquid separation/mass mapping technique | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Chemistry, University of Michigan, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Department of Chemistry, University of Michigan, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Department of Chemistry, University of Michigan, Ann Arbor, MI, USA ; Department of Surgery, University of Michigan Medical Center, Ann Arbor, MI, USA ; Comprehensive Cancer Center, University of Michigan Medical Center, Ann Arbor, MI, USA ; Department of Surgery, The University of Michigan Medical Center, MSRB1, 1150 West Medical Center Drive, Ann Arbor, MI 48109-0656, USA Fax: +1-734-615-8108 | en_US |
dc.contributor.affiliationother | Breast Cancer Program, Barbara Ann Karmanos Cancer Institute, Detroit, MI, USA ; Department of Pathology, Wayne State University School of Medicine, Detroit, MI, USA | en_US |
dc.contributor.affiliationother | Breast Cancer Program, Barbara Ann Karmanos Cancer Institute, Detroit, MI, USA ; Department of Pathology, Wayne State University School of Medicine, Detroit, MI, USA | en_US |
dc.contributor.affiliationother | Breast Cancer Program, Barbara Ann Karmanos Cancer Institute, Detroit, MI, USA | en_US |
dc.contributor.affiliationother | Eprogen, Darien, IL, USA | en_US |
dc.identifier.pmid | 16767785 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/55250/1/3847_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/pmic.200500195 | en_US |
dc.identifier.source | PROTEOMICS | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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