Obscurin is required for the lateral alignment of striated myofibrils in zebrafish
dc.contributor.author | Raeker, Maide Ö. | en_US |
dc.contributor.author | Su, Fengyun | en_US |
dc.contributor.author | Geisler, Sarah B. | en_US |
dc.contributor.author | Borisov, Andrei B. | en_US |
dc.contributor.author | Kontrogianni-Konstantopoulos, Aikaterini | en_US |
dc.contributor.author | Lyons, Susan E. | en_US |
dc.contributor.author | Russell, Mark W. | en_US |
dc.date.accessioned | 2007-09-18T19:21:48Z | |
dc.date.available | 2007-09-18T19:21:48Z | |
dc.date.issued | 2006-08 | en_US |
dc.identifier.citation | Raeker, Maide Ö.; Su, Fengyun; Geisler, Sarah B.; Borisov, Andrei B.; Kontrogianni-Konstantopoulos, Aikaterini; Lyons, Susan E.; Russell, Mark W. (2006). "Obscurin is required for the lateral alignment of striated myofibrils in zebrafish." Developmental Dynamics 235(8): 2018-2029. <http://hdl.handle.net/2027.42/55797> | en_US |
dc.identifier.issn | 1058-8388 | en_US |
dc.identifier.issn | 1097-0177 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/55797 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=16779859&dopt=citation | en_US |
dc.description.abstract | Obscurin/obscurin-MLCK is a giant sarcomere-associated protein with multiple isoforms whose interactions with titin and small ankyrin-1 suggest that it has an important role in myofibril assembly, structural support, and the sarcomeric alignment of the sarcoplasmic reticulum. In this study, we characterized the zebrafish orthologue of obscurin and examined its role in striated myofibril assembly. Zebrafish obscurin was expressed in the somites and central nervous system by 24 hours post-fertilization (hpf) and in the heart by 48 hpf. Depletion of obscurin using two independent morpholino antisense oligonucleotides resulted in diminished numbers and marked disarray of skeletal myofibrils, impaired lateral alignment of adjacent myofibrils, disorganization of the sarcoplasmic reticulum, somite segmentation defects, and abnormalities of cardiac structure and function. This is the first demonstration that obscurin is required for vertebrate cardiac and skeletal muscle development. The diminished capacity to generate and organize new myofibrils in response to obscurin depletion suggests that it may have a vital role in the causation of or adaptation to cardiac and skeletal myopathies. Developmental Dynamics 235:2018–2029, 2006. © 2006 Wiley-Liss, Inc. | en_US |
dc.format.extent | 1135270 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Wiley-Liss, Inc. | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Cell & Developmental Biology | en_US |
dc.title | Obscurin is required for the lateral alignment of striated myofibrils in zebrafish | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Pediatrics | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, Michigan ; Associate Professor of Pediatrics and Communicable Diseases, Division of Pediatric Cardiology, University of Michigan, L1242 Women's Hospital/Box 0204, 1500 E. Medical Center Dr., Ann Arbor, MI 48109 | en_US |
dc.contributor.affiliationother | Department of Physiology, University of Maryland School of Medicine, Baltimore, Maryland | en_US |
dc.identifier.pmid | 16779859 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/55797/1/20812_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/dvdy.20812 | en_US |
dc.identifier.source | Developmental Dynamics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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