Detection of mammalian microRNA expression by in situ hybridization with RNA oligonucleotides
dc.contributor.author | Deo, Monika | en_US |
dc.contributor.author | Yu, Jenn-Yah | en_US |
dc.contributor.author | Chung, Kwan-Ho | en_US |
dc.contributor.author | Tippens, Melissa | en_US |
dc.contributor.author | Turner, David L. | en_US |
dc.date.accessioned | 2007-09-18T19:21:59Z | |
dc.date.available | 2007-09-18T19:21:59Z | |
dc.date.issued | 2006-09 | en_US |
dc.identifier.citation | Deo, Monika; Yu, Jenn-Yah; Chung, Kwan-Ho; Tippens, Melissa; Turner, David L. (2006). "Detection of mammalian microRNA expression by in situ hybridization with RNA oligonucleotides." Developmental Dynamics 235(9): 2538-2548. <http://hdl.handle.net/2027.42/55798> | en_US |
dc.identifier.issn | 1058-8388 | en_US |
dc.identifier.issn | 1097-0177 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/55798 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=16736490&dopt=citation | en_US |
dc.description.abstract | We have developed an in situ hybridization procedure for the detection of microRNAs (miRNAs) in tissue sections from mouse embryos and adult organs. The method uses highly specific washing conditions for RNA oligonucleotide probes conjugated to a fluorescein hapten. We show that this method detects predominantly mature miRNAs rather than the miRNA precursors or primary transcripts. We have determined expression patterns for several miRNAs expressed in the developing and adult nervous system, including miR-124a, miR-9, miR-92, and miR-204. Whereas miR-124a is expressed in neurons, miR-9 is expressed in neural progenitors and some neurons, and miR-204 is expressed in the choroid plexus, retinal pigment epithelium, and ciliary body. miR-204 is located in an intron of the TRPM3 gene, and the TRPM3 mRNA is coexpressed with miR-204 in the choroid plexus. We also find that primary transcripts for miR-124a and miR-9 genes are expressed in patterns similar to their respective mature miRNAs. The ability to visualize expression of specific miRNAs in embryos and tissues should aid studies on miRNA function. Developemental Dynamics 235:2538–2548, 2006. © 2006 Wiley-Liss, Inc. | en_US |
dc.format.extent | 913611 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Wiley-Liss, Inc. | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Cell & Developmental Biology | en_US |
dc.title | Detection of mammalian microRNA expression by in situ hybridization with RNA oligonucleotides | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Pediatrics | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Molecular and Behavioral Neuroscience Institute, University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Molecular and Behavioral Neuroscience Institute, University of Michigan, Ann Arbor, Michigan ; Program in Neuroscience, University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Molecular and Behavioral Neuroscience Institute, University of Michigan, Ann Arbor, Michigan ; Program in Neuroscience, University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Molecular and Behavioral Neuroscience Institute, University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Molecular and Behavioral Neuroscience Institute, University of Michigan, Ann Arbor, Michigan ; Program in Neuroscience, University of Michigan, Ann Arbor, Michigan ; Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan ; Molecular and Behavioral Neuroscience Institute, University of Michigan, 5062 BSRB, 109 Zina Pitcher Place, Ann Arbor, MI 48109-2200 | en_US |
dc.identifier.pmid | 16736490 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/55798/1/20847_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/dvdy.20847 | en_US |
dc.identifier.source | Developmental Dynamics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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