Neoadjuvant intratumoral cytokine-loaded microspheres are superior to postoperative autologous cellular vaccines in generating systemic anti-tumor immunity
dc.contributor.author | Arora, Alisha | en_US |
dc.contributor.author | Su, Gang | en_US |
dc.contributor.author | Mathiowitz, Edith | en_US |
dc.contributor.author | Reineke, Joshua | en_US |
dc.contributor.author | Chang, Alfred E. | en_US |
dc.contributor.author | Sabel, Michael S. | en_US |
dc.date.accessioned | 2007-09-20T16:41:45Z | |
dc.date.available | 2008-01-03T16:18:46Z | en_US |
dc.date.issued | 2006-10-01 | en_US |
dc.identifier.citation | Arora, Alisha; Su, Gang; Mathiowitz, Edith; Reineke, Joshua; Chang, Alfred E.; Sabel, Michael S. (2006). "Neoadjuvant intratumoral cytokine-loaded microspheres are superior to postoperative autologous cellular vaccines in generating systemic anti-tumor immunity." Journal of Surgical Oncology 94(5): 403-412. <http://hdl.handle.net/2027.42/55822> | en_US |
dc.identifier.issn | 0022-4790 | en_US |
dc.identifier.issn | 1096-9098 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/55822 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=16967445&dopt=citation | en_US |
dc.description.abstract | Background Sustained intratumoral cytokine release using poly-lactic acid microspheres (PLAMs) can induce a systemic immune response, shifting immunotherapy to the neoadjuvant setting. Methods C57BL6 mice with established B16 melanomas underwent a single intralesional injection of IL-12, TNF-Α or GM-CSF PLAM, alone or in combination. Tumor draining lymph nodes (TDLN) and spleens were assessed for a specific anti-tumor response by IFNΓ release assay and ELISPOT. Results Intralesional injection of TNF-Α, alone or in combination, resulted in significant tumor ablation. The induction of tumor specific reactive T-cells in the TDLN was greatest with IL-12 and TNF-Α. Only mice treated with IL-12 and TNF-Α demonstrated a substantial T-cell response in cultured splenocytes. This combination resulted in a significant reduction in new tumors after re-challenge. Adjuvant therapy, using irradiated B16 cells in combination with equivalent doses of IL-12 and TNF-Α, failed to generate a similar T-cell response or prevent re-challenge. Conclusions Intratumoral IL-12 and TNF-Α loaded PLAM leads to both local eradication of tumor and the induction of specific anti-tumor T-cells in the lymph nodes and spleens, resulting in memory immune response. Neoadjuvant treatment was significantly superior to postoperative autologous cellular vaccines using IL-12 and TNF-Α PLAM. J. Surg. Oncol. 2006;94:403–412. © 2006 Wiley-Liss, Inc. | en_US |
dc.format.extent | 302090 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Cancer Research, Oncology and Pathology | en_US |
dc.title | Neoadjuvant intratumoral cytokine-loaded microspheres are superior to postoperative autologous cellular vaccines in generating systemic anti-tumor immunity | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Oncology and Hematology | en_US |
dc.subject.hlbsecondlevel | Surgery and Anesthesiology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Division of Surgical Oncology, University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Division of Surgical Oncology, University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Division of Surgical Oncology, University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Division of Surgical Oncology, University of Michigan, Ann Arbor, Michigan ; 3304 Cancer Center, 1500 East Medical Center Drive, Ann Arbor, MI 48109-0932. Fax: +734-647-9647. | en_US |
dc.contributor.affiliationother | Department of Molecular Pharmacology, Physiology and Biotechnology, Brown University, Providence, Rhode Island | en_US |
dc.contributor.affiliationother | Department of Molecular Pharmacology, Physiology and Biotechnology, Brown University, Providence, Rhode Island | en_US |
dc.identifier.pmid | 16967445 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/55822/1/20572_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/jso.20572 | en_US |
dc.identifier.source | Journal of Surgical Oncology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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