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Temporally regulated delivery of VEGF in vitro and in vivo

dc.contributor.authorEnnett, Alessandra B.en_US
dc.contributor.authorKaigler, Darnellen_US
dc.contributor.authorMooney, David J.en_US
dc.date.accessioned2007-09-20T17:39:50Z
dc.date.available2008-01-03T16:18:58Zen_US
dc.date.issued2006-10en_US
dc.identifier.citationEnnett, Alessandra B.; Kaigler, Darnell; Mooney, David J. (1)."Temporally regulated delivery of VEGF in vitro and in vivo ." Journal of Biomedical Materials Research Part A 79A: 176-184. <http://hdl.handle.net/2027.42/55823>en_US
dc.identifier.issn1549-3296en_US
dc.identifier.issn1552-4965en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/55823
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=16788907&dopt=citationen_US
dc.description.abstractThe exposure duration and tissue distribution will likely dictate the success of vascular endothelial growth factor (VEGF) in therapeutic angiogenesis. We hypothesized that these variables can be regulated via the manner in which the VEGF is incorporated into polymer constructs (formed with a gas foaming technique) used for its delivery. VEGF was incorporated directly into poly(lactide- co -glycolide) (PLG) scaffolds or pre-encapsulated in PLG microspheres used to fabricate scaffolds. Protein release kinetics and tissue distribution were determined using iodinated VEGF. VEGF was positioned predominantly adjacent to scaffold pores when incorporated directly and was released rapidly (40–60% in 5 days). Pre-encapsulation led to the VEGF being more deeply embedded and resulted in a delayed release. Alterations in polymer composition, scaffold size, and matrix composition generated minor variations in release kinetics. In vivo , the released VEGF generated local protein concentrations above 10 ng/mL at distances up to 2 cm from the implant site for the 21 days of the experiment, with negligible release into the systemic circulation, and significantly enhanced local angiogenesis. These data indicate that VEGF can be administered in a sustained and localized fashion in vivo , and the timing of VEGF delivery can be altered with the mechanism of incorporation into polymer scaffolds used for its delivery. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006en_US
dc.format.extent627100 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.publisherWiley Subscription Services, Inc., A Wiley Companyen_US
dc.subject.otherChemistryen_US
dc.subject.otherPolymer and Materials Scienceen_US
dc.titleTemporally regulated delivery of VEGF in vitro and in vivoen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelBiomedical Engineeringen_US
dc.subject.hlbtoplevelEngineeringen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDivision of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts ; Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michiganen_US
dc.contributor.affiliationumDepartment of Biologic and Materials Sciences, University of Michigan, Ann Arbor, Michiganen_US
dc.contributor.affiliationotherDivision of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts ; Division of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusettsen_US
dc.identifier.pmid16788907en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/55823/1/30771_ftp.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1002/jbm.a.30771en_US
dc.identifier.sourceJournal of Biomedical Materials Research Part Aen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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