Role of aggrecanase 1 in Lyme arthritis
dc.contributor.author | Behera, Aruna K. | en_US |
dc.contributor.author | Hildebrand, Ethan | en_US |
dc.contributor.author | Szafranski, Jon | en_US |
dc.contributor.author | Hung, Han-Hwa | en_US |
dc.contributor.author | Grodzinsky, Alan J. | en_US |
dc.contributor.author | Lafyatis, Robert | en_US |
dc.contributor.author | Koch, Alisa E. | en_US |
dc.contributor.author | Kalish, Robert | en_US |
dc.contributor.author | Perides, George | en_US |
dc.contributor.author | Steere, Allen C. | en_US |
dc.contributor.author | Hu, Linden T. | en_US |
dc.date.accessioned | 2007-09-20T17:40:25Z | |
dc.date.available | 2008-01-03T16:19:07Z | en_US |
dc.date.issued | 2006-10 | en_US |
dc.identifier.citation | Behera, Aruna K.; Hildebrand, Ethan; Szafranski, Jon; Hung, Han-Hwa; Grodzinsky, Alan J.; Lafyatis, Robert; Koch, Alisa E.; Kalish, Robert; Perides, George; Steere, Allen C.; Hu, Linden T. (2006). "Role of aggrecanase 1 in Lyme arthritis." Arthritis & Rheumatism 54(10): 3319-3329. <http://hdl.handle.net/2027.42/55825> | en_US |
dc.identifier.issn | 0004-3591 | en_US |
dc.identifier.issn | 1529-0131 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/55825 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=17009305&dopt=citation | en_US |
dc.description.abstract | Objective Arthritis is one of the hallmarks of late-stage Lyme disease. Previous studies have shown that infection with Borrelia burgdorferi , the causative agent of Lyme disease, results in degradation of proteoglycans and collagen in cartilage. B burgdorferi do not appear to produce any exported proteases capable of digesting proteoglycans and collagen, but instead, induce and activate host proteases, such as matrix metalloproteinases (MMPs), which results in cartilage degradation. The role of aggrecanases in Lyme arthritis has not yet been determined. We therefore sought to delineate the contribution of aggrecanases to joint destruction in Lyme arthritis. Methods We examined the expression patterns of aggrecanases 1 and 2 (ADAMTS 4 and 5, respectively) in B burgdorferi –infected primary human chondrocyte cell cultures, in synovial fluid samples from patients with active Lyme arthritis, and in the joints of mice by real-time quantitative reverse transcription–polymerase chain reaction and immunoblotting techniques. Bovine cartilage explants were used to determine the role of aggrecanases in B burgdorferi –induced cartilage degradation. Results ADAMTS-4, but not ADAMTS-5, was induced in human chondrocytes infected with B burgdorferi . The active forms of ADAMTS-4 were increased in synovial fluid samples from patients with active Lyme arthritis and were elevated in the joints of mice infected with B burgdorferi . Using cartilage explant models of Lyme arthritis, it appeared that the cleavage of aggrecan was predominantly mediated by “aggrecanases” rather than MMPs. Conclusion The induction of ADAMTS-4 by B burgdorferi results in the cleavage of aggrecan, which may be an important first step that leads to permanent degradation of cartilage. | en_US |
dc.format.extent | 471674 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.title | Role of aggrecanase 1 in Lyme arthritis | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Geriatrics | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Veterans Affairs Healthcare System, and University of Michigan Medical School, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationother | Tupper Research Institute, Tufts University School of Medicine, Boston, Massachusetts | en_US |
dc.contributor.affiliationother | Tupper Research Institute, Tufts University School of Medicine, Boston, Massachusetts | en_US |
dc.contributor.affiliationother | Massachusetts Institute of Technology, Cambridge, Massachusetts | en_US |
dc.contributor.affiliationother | Massachusetts Institute of Technology, Cambridge, Massachusetts | en_US |
dc.contributor.affiliationother | Massachusetts Institute of Technology, Cambridge, Massachusetts | en_US |
dc.contributor.affiliationother | Boston University School of Medicine, Boston, Massachusetts | en_US |
dc.contributor.affiliationother | Tupper Research Institute, Tufts University School of Medicine, Boston, Massachusetts | en_US |
dc.contributor.affiliationother | Tupper Research Institute, Tufts University School of Medicine, Boston, Massachusetts | en_US |
dc.contributor.affiliationother | Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts | en_US |
dc.contributor.affiliationother | Tupper Research Institute, Tufts University School of Medicine, Boston, Massachusetts ; New England Medical Center, Box 41, 750 Washington Street, Boston, MA 02111 | en_US |
dc.identifier.pmid | 17009305 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/55825/1/22128_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/art.22128 | en_US |
dc.identifier.source | Arthritis & Rheumatism | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.