Substrate mineralization stimulates focal adhesion contact redistribution and cell motility of bone marrow stromal cells
dc.contributor.author | Leonova, Elena V. | en_US |
dc.contributor.author | Pennington, Keith E. | en_US |
dc.contributor.author | Krebsbach, Paul H. | en_US |
dc.contributor.author | Kohn, David H. | en_US |
dc.date.accessioned | 2007-09-20T17:46:32Z | |
dc.date.available | 2008-01-03T16:20:31Z | en_US |
dc.date.issued | 2006-11 | en_US |
dc.identifier.citation | Leonova, Elena V.; Pennington, Keith E.; Krebsbach, Paul H.; Kohn, David H. (2)."Substrate mineralization stimulates focal adhesion contact redistribution and cell motility of bone marrow stromal cells." Journal of Biomedical Materials Research Part A 79A: 263-270. <http://hdl.handle.net/2027.42/55848> | en_US |
dc.identifier.issn | 1549-3296 | en_US |
dc.identifier.issn | 1552-4965 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/55848 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=16817221&dopt=citation | en_US |
dc.description.abstract | Understanding the mechanisms of substrate based control of cell function is critical to the design of biomaterials. Cells interact with their extracellular matrix through cell adhesion contacts. We have previously described the self assembly of bone-like mineral onto an organic template and have shown that these biomimetic surfaces lead to an increased volume fraction of bone regenerated in vivo . In the present study, we compared the distribution of cell adhesion contacts, cell spreading, and cell motility of murine bone marrow stromal cells (BMSC) on mineralized vs. nonmineralized substrates. We developed a new approach for quantification of cell-material interactions and demonstrated that cell adhesion contacts on mineralized substrates were distributed throughout the cell surface contacting the substrate, whereas on nonmineralized substrates cell adhesion contacts were present near the cell periphery. We propose that mineralized substrates stimulate the predominant expression of fibrillar contacts, and nonmineralized substrates stimulate expression of focal adhesion contacts. Cell motility assays with colloidal gold demonstrated a statistically significant decrease in the average phagokinetic index of migrating cells on mineralized vs. nonmineralized substrates after 90 min of cell seeding. We propose that the physical–chemical properties of the substrate, altered by mineralization, cause expression of specific types of cell contacts and, as a result, modify molecular mechanisms responsible for cell spreading, motility, and possibly differentiation. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006 | en_US |
dc.format.extent | 370263 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Polymer and Materials Science | en_US |
dc.title | Substrate mineralization stimulates focal adhesion contact redistribution and cell motility of bone marrow stromal cells | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Biomedical Engineering | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biologic and Materials Sciences, University of Michigan, Ann Arbor, Michigan 48109-1078 | en_US |
dc.contributor.affiliationum | Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan 48109-2099 | en_US |
dc.contributor.affiliationum | Department of Biologic and Materials Sciences, University of Michigan, Ann Arbor, Michigan 48109-1078 ; Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan 48109-2099 | en_US |
dc.contributor.affiliationum | Department of Biologic and Materials Sciences, University of Michigan, Ann Arbor, Michigan 48109-1078 ; Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan 48109-2099 ; Department of Biologic and Materials Sciences, University of Michigan, Ann Arbor, Michigan 48109-1078 | en_US |
dc.identifier.pmid | 16817221 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/55848/1/30786_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/jbm.a.30786 | en_US |
dc.identifier.source | Journal of Biomedical Materials Research Part A | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.