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dc.contributor.authorXia, Yong-Meien_US
dc.contributor.authorHua, Zhi-Shanen_US
dc.contributor.authorSrivannavit, Onnopen_US
dc.contributor.authorOzel, Ayse Bilgeen_US
dc.contributor.authorGulari, Erdoganen_US
dc.date.accessioned2007-09-20T17:54:21Z
dc.date.available2008-04-03T18:44:50Zen_US
dc.date.issued2007-01en_US
dc.identifier.citationXia, Yong-Mei; Hua, Zhi-Shan; Srivannavit, Onnop; Ozel, Ayse Bilge; Gulari, Erdogan (2007). "Minimizing the surface effect of PDMS–glass microchip on polymerase chain reaction by dynamic polymer passivation." Journal of Chemical Technology & Biotechnology 82(1): 33-38. <http://hdl.handle.net/2027.42/55877>en_US
dc.identifier.issn0268-2575en_US
dc.identifier.issn1097-4660en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/55877
dc.description.abstractPolydimethyl siloxane (PDMS)–glass microchip has a very strong surface effect on polymerase chain reaction (PCR), leading to a very poor PCR yield. In the work reported here, practical dynamic passivation of surfaces of PDMS–glass microchip using polyethylene glycol (PEG) or polyvinylpyrrolidone (PVP) was achieved using a conventional thermocycler. The passivation procedure was cost-effective and easy to conduct. The effects of polymer molecular weight and polymer concentration on tube PCR efficiency were investigated primarily to prescreen out suitable polymers and polymer concentrations in the PCR mixture. The result from tube PCR indicated that both PEG and PVP could affect the performance of Taq polymerase. A final concentration of 0.025% (w/v) or 0.4% (w/v) polymer in the PCR mixture can enhance the tube PCR, while 1% (w/v) polymer was found to inhibit the reaction. PEG was more effective in tube PCR, although PVP performed better in chip PCR. Instead of employing the polymer directly in the PCR mixture, i.e. the conventional in situ passivation approach, another approach of dynamic passivation by pre-injecting polymers into the microchip achieved better performance. The efficiency of pre-passivation was found to follow the order: PVP10000>PVP55000, PEG8000> PEG10000>PEG400. After pre-passivation with PVP10000, PVP55000 and PEG8000, the PCR efficiency can recover to 93%, 86% and 83%, respectively, of that obtained from tube PCR. Copyright © 2006 Society of Chemical Industryen_US
dc.format.extent272905 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.publisherJohn Wiley & Sons, Ltd.en_US
dc.subject.otherChemistryen_US
dc.subject.otherBiochemistry and Biotechnologyen_US
dc.titleMinimizing the surface effect of PDMS–glass microchip on polymerase chain reaction by dynamic polymer passivationen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelChemical Engineeringen_US
dc.subject.hlbsecondlevelBiomedical Engineeringen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelEngineeringen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumSchool of Chemical and Material Engineering, Southern Yangtze University, 170# Huihe Road, Wuxi, Jiangsu 214036, China ; Department of Chemical Engineering, University of Michigan, 3085 H.H. Dow, Ann Arbor, MI 48109, USA ; School of Chemical and Material Engineering, Southern Yangtze University, Wuxi, Jiangsu 214036, Chinaen_US
dc.contributor.affiliationumDepartment of Chemical Engineering, University of Michigan, 3085 H.H. Dow, Ann Arbor, MI 48109, USAen_US
dc.contributor.affiliationumDepartment of Chemical Engineering, University of Michigan, 3085 H.H. Dow, Ann Arbor, MI 48109, USAen_US
dc.contributor.affiliationumDepartment of Chemical Engineering, University of Michigan, 3085 H.H. Dow, Ann Arbor, MI 48109, USAen_US
dc.contributor.affiliationumDepartment of Chemical Engineering, University of Michigan, 3085 H.H. Dow, Ann Arbor, MI 48109, USAen_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/55877/1/1631_ftp.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1002/jctb.1631en_US
dc.identifier.sourceJournal of Chemical Technology & Biotechnologyen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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