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Effect of Immobilized Nerve Growth Factor on Conductive Polymers: Electrical Properties and Cellular Response This work was supported in part by the National Institutes of Health (NINDS-N01-NS-1-2338), the National Science Foundation (DMR-0084304, DMR-0518079), the NASA BioScience and Engineering Institute, the Undergraduate Research Opportunity Program, the training program in Regenerative Sciences, and the University of Michigan, College of Engineering.

dc.contributor.authorKim, D. -H.en_US
dc.contributor.authorRichardson-Burns, S.  m.en_US
dc.contributor.authorHendricks, Jeffrey L.en_US
dc.contributor.authorSequera, C.en_US
dc.date.accessioned2007-09-20T18:05:59Z
dc.date.available2008-04-03T18:47:44Zen_US
dc.date.issued2007-01-05en_US
dc.identifier.citationKim, D.-H.; Richardson-Burns, S. M.; Hendricks, J. L.; Sequera, C. (2007). "Effect of Immobilized Nerve Growth Factor on Conductive Polymers: Electrical Properties and Cellular Response This work was supported in part by the National Institutes of Health (NINDS-N01-NS-1-2338), the National Science Foundation (DMR-0084304, DMR-0518079), the NASA BioScience and Engineering Institute, the Undergraduate Research Opportunity Program, the training program in Regenerative Sciences, and the University of Michigan, College of Engineering. ." Advanced Functional Materials 17(1): 79-86. <http://hdl.handle.net/2027.42/55920>en_US
dc.identifier.issn1616-301Xen_US
dc.identifier.issn1616-3028en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/55920
dc.description.abstractThe use of biologically active dopants in conductive polymers allows the polymer to be tailored for specific applications. The incorporation of nerve growth factor (NGF) as a co-dopant in the electrochemical deposition of conductive polymers is evaluated for its ability to elicit specific biological interactions with neurons. The electrochemical properties of the NGF-modified conducting polymers are studied by impedance spectroscopy and cyclic voltammetry. Impedance measurements at the neurobiologically important frequency of 1 kHz reveal that the minimum impedance of the NGF-modified polypyrrole (PPy) film, 15 kω, is lower than the minimum impedance of peptide-modified PPy film (360 kω). Similar results are found with NGF-modified poly(3,4-ethylene dioxythiophene) (PEDOT). The microstructure of the conductive polymer films is characterized by optical microscopy and electron microscopy and indicates that the NGF-functionalized polymer surface topology is similar to that of the unmodified polymer film. Optical and fluorescence microscopy reveal that PC-12 (rat pheochromacytoma) cells adhered to the NGF-modified substrate and extended neurites on both PPy and PEDOT, indicating that the NGF in the polymer film is biologically active. Taken together these data indicate that the incorporation of NGF can modify the biological interactions of the electrode without compromising the conductive properties or the morphology of the polymeric film.en_US
dc.format.extent556559 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.publisherWILEY-VCH Verlagen_US
dc.subject.otherChemistryen_US
dc.subject.otherPolymer and Materials Scienceen_US
dc.titleEffect of Immobilized Nerve Growth Factor on Conductive Polymers: Electrical Properties and Cellular Response This work was supported in part by the National Institutes of Health (NINDS-N01-NS-1-2338), the National Science Foundation (DMR-0084304, DMR-0518079), the NASA BioScience and Engineering Institute, the Undergraduate Research Opportunity Program, the training program in Regenerative Sciences, and the University of Michigan, College of Engineering.en_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelEngineering (General)en_US
dc.subject.hlbsecondlevelMaterials Science and Engineeringen_US
dc.subject.hlbtoplevelEngineeringen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Materials Science and Engineering, The University of Michigan, Ann Arbor, MI 48109, USA ; University of Michigan, Regenerative Sciences Training Program, Ann Arbor, MI 48109, USAen_US
dc.contributor.affiliationumDepartment of Biomedical Engineering, The University of Michigan, Ann Arbor, MI 48109, USAen_US
dc.contributor.affiliationumDepartment of Chemical Engineering, The University of Michigan, Ann Arbor, MI 48109, USAen_US
dc.contributor.affiliationotherDepartment of Biomedical Engineering, Duke University, Durham, NC 27708-0281, USAen_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/55920/1/79_ftp.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1002/adfm.200500594en_US
dc.identifier.sourceAdvanced Functional Materialsen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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