A proteomic analysis of Psychrobacter articus 273-4 adaptation to low temperature and salinity using a 2-D liquid mapping approach
dc.contributor.author | Zheng, Suping | en_US |
dc.contributor.author | Ponder, Monica A. | en_US |
dc.contributor.author | Shih, Janice Yang Jen | en_US |
dc.contributor.author | Tiedje, James M. | en_US |
dc.contributor.author | Thomashow, Michael F. | en_US |
dc.contributor.author | Lubman, David M. | en_US |
dc.date.accessioned | 2007-09-20T18:10:52Z | |
dc.date.available | 2008-04-03T18:49:26Z | en_US |
dc.date.issued | 2007-02 | en_US |
dc.identifier.citation | Zheng, Suping; Ponder, Monica A.; Shih, Janice Yang Jen; Tiedje, James M.; Thomashow, Michael F.; Lubman, David M. (2007). "A proteomic analysis of Psychrobacter articus 273-4 adaptation to low temperature and salinity using a 2-D liquid mapping approach." Electrophoresis 28(3): 467-488. <http://hdl.handle.net/2027.42/55939> | en_US |
dc.identifier.issn | 0173-0835 | en_US |
dc.identifier.issn | 1522-2683 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/55939 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=17177241&dopt=citation | en_US |
dc.description.abstract | Psychrobacter 273-4 was isolated from a 20 000–40 000–year-old Siberian permafrost core, which is characterized by low temperature, low water activity, and high salinity. To explore how 273-4 survives in the permafrost environment, proteins in four 273-4 samples cultured at 4 and 22°C in media with and without 5% 14sodium chloride were profiled and comparatively studied using 2-D HPLC and MS. The method used herein involved fractionation via a pH gradient using chromatofocusing followed by nonporous silica 14(NPS) RP-HPLC and on-line electrospray mass mapping. It was observed that 33 14proteins were involved in the adaptation to low temperature in the cells grown in the nonsaline media while there were only 14 proteins involved in the saline media. There were 45 14proteins observed differentially expressed in response to salt at 22°C while there were 22 14proteins at 4°C. In addition, 5% 14NaCl and 4°C showed a combination effect on protein expression. A total of 56 14proteins involved in the adaptation to low temperature and salt were identified using MS and database searching. The differentially expressed proteins were classified into different functional categories where the response of the regulation system to stress appears to be very elaborate. The evidence shows that the adaptation of 273-4 is based primarily on the control of translation and transcription, the synthesis of proteins (chaperones) to facilitate RNA and protein folding, and the regulation of metabolic pathways. | en_US |
dc.format.extent | 520153 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | WILEY-VCH Verlag | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Biochemistry and Biotechnology | en_US |
dc.title | A proteomic analysis of Psychrobacter articus 273-4 adaptation to low temperature and salinity using a 2-D liquid mapping approach | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Chemistry, University of Michigan, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, University of Michigan, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Department of Chemistry, University of Michigan, Ann Arbor, MI, USA ; Department of Surgery, University of Michigan Medical Center, Ann Arbor, MI, USA ; Department of Surgery, The University of Michigan, 1150 West Medical Center Drive, Building MSRB1, Rm A510B, Ann Arbor, MI 48109, USA Fax: +1-734-615-2088 | en_US |
dc.contributor.affiliationother | Center for Genomic and Evolutionary Studies on Microbial Life at Low Temperature, Michigan State University, East Lansing, MI, USA ; Center for Microbial Ecology, Michigan State University, East Lansing, MI, USA | en_US |
dc.contributor.affiliationother | Center for Genomic and Evolutionary Studies on Microbial Life at Low Temperature, Michigan State University, East Lansing, MI, USA ; Center for Microbial Ecology, Michigan State University, East Lansing, MI, USA | en_US |
dc.contributor.affiliationother | Center for Genomic and Evolutionary Studies on Microbial Life at Low Temperature, Michigan State University, East Lansing, MI, USA ; Center for Microbial Ecology, Michigan State University, East Lansing, MI, USA | en_US |
dc.identifier.pmid | 17177241 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/55939/1/467_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/elps.200600173 | en_US |
dc.identifier.source | Electrophoresis | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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