Raman spectral signatures of mouse mammary tissue and associated lymph nodes: normal, tumor and mastitis

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dc.contributor.author Thakur, Jagdish S. en_US
dc.contributor.author Dai, Houbei en_US
dc.contributor.author Serhatkulu, Gulay K. en_US
dc.contributor.author Naik, Ratna en_US
dc.contributor.author Naik, Vaman M. en_US
dc.contributor.author Cao, Alex en_US
dc.contributor.author Pandya, Abhilash en_US
dc.contributor.author Auner, Gregory W. en_US
dc.contributor.author Rabah, Rajah en_US
dc.contributor.author Klein, Michael D. en_US
dc.contributor.author Freeman, Carl en_US
dc.date.accessioned 2007-09-20T18:12:53Z
dc.date.available 2008-04-03T18:49:56Z en_US
dc.date.issued 2007-02 en_US
dc.identifier.citation Thakur, Jagdish S.; Dai, Houbei; Serhatkulu, Gulay K.; Naik, Ratna; Naik, Vaman M.; Cao, Alex; Pandya, Abhilash; Auner, Gregory W.; Rabah, Rajah; Klein, Michael D.; Freeman, Carl (2007). "Raman spectral signatures of mouse mammary tissue and associated lymph nodes: normal, tumor and mastitis." Journal of Raman Spectroscopy 38(2): 127-134. <http://hdl.handle.net/2027.42/55947> en_US
dc.identifier.issn 0377-0486 en_US
dc.identifier.issn 1097-4555 en_US
dc.identifier.uri http://hdl.handle.net/2027.42/55947
dc.description.abstract Raman spectroscopy involves the interaction of light with the molecular vibrations and therefore can provide information about molecular structure, tissue composition and changes in its environment. We explored whether Raman spectroscopy can reliably distinguish mammary tumors from normal mammary tissues and other pathological states in mice. We analyzed a large number of Raman spectra from the tumor and normal mammary glands of mice injected with 4T1 tumor cells, which were collected using a high-resolution (less than 4 cm −1 ) Raman spectrometer at a fixed (785 nm) laser excitation wavelength and with 60 mW of laser power. The spectra of normal and tumor mammary glands showed consistent differences in the intensity of certain Raman bands and loss of some bands in the tumor spectra. Multivariate statistical methods—principal component analysis (PCA) and discriminant functional analysis (DFA)—were used to separate the data into different groups of mammary tumors, mastitis, lymph nodes contralateral and tumor-cell-injected sides, and normal contralateral and tumor-cell-injected sides. We demonstrate that this spectroscopic technique has the feasibility of discriminating tumor and mastitis from normal tissues and other pathological states in a short period of time and may detect tumor transformation earlier than the standard histological examination stage. Copyright © 2006 John Wiley & Sons, Ltd. en_US
dc.format.extent 1006758 bytes
dc.format.extent 3118 bytes
dc.format.mimetype application/pdf
dc.format.mimetype text/plain
dc.publisher John Wiley & Sons, Ltd. en_US
dc.subject.other Chemistry en_US
dc.subject.other Analytical Chemistry and Spectroscopy en_US
dc.title Raman spectral signatures of mouse mammary tissue and associated lymph nodes: normal, tumor and mastitis en_US
dc.type Article en_US
dc.rights.robots IndexNoFollow en_US
dc.subject.hlbsecondlevel Biological Chemistry en_US
dc.subject.hlbsecondlevel Chemistry en_US
dc.subject.hlbsecondlevel Physics en_US
dc.subject.hlbtoplevel Health Sciences en_US
dc.subject.hlbtoplevel Science en_US
dc.description.peerreviewed Peer Reviewed en_US
dc.contributor.affiliationum Department of Natural Sciences, University of Michigan-Dearborn, Dearborn, MI 48128, USA en_US
dc.contributor.affiliationother Department of Electrical and Computer Engineering, Wayne State University, Detroit, MI 48202, USA ; Department of Electrical and Computer Engineering, Wayne State University, Detroit, MI 48202, USA. en_US
dc.contributor.affiliationother Department of Physics and Astronomy, Wayne State University, Detroit, MI 48202, USA en_US
dc.contributor.affiliationother Department of Electrical and Computer Engineering, Wayne State University, Detroit, MI 48202, USA en_US
dc.contributor.affiliationother Department of Physics and Astronomy, Wayne State University, Detroit, MI 48202, USA en_US
dc.contributor.affiliationother Department of Electrical and Computer Engineering, Wayne State University, Detroit, MI 48202, USA en_US
dc.contributor.affiliationother Department of Electrical and Computer Engineering, Wayne State University, Detroit, MI 48202, USA en_US
dc.contributor.affiliationother Department of Electrical and Computer Engineering, Wayne State University, Detroit, MI 48202, USA en_US
dc.contributor.affiliationother Departments of Surgery (Pediatric Surgery) and Pathology, Wayne State University School of Medicine and the Children's Hospital of Michigan, Detroit, MI 48201, USA en_US
dc.contributor.affiliationother Departments of Surgery (Pediatric Surgery) and Pathology, Wayne State University School of Medicine and the Children's Hospital of Michigan, Detroit, MI 48201, USA en_US
dc.contributor.affiliationother Department of Biological Sciences, Wayne State University, Detroit, MI 48202, USA en_US
dc.description.bitstreamurl http://deepblue.lib.umich.edu/bitstream/2027.42/55947/1/1565_ftp.pdf en_US
dc.identifier.doi http://dx.doi.org/10.1002/jrs.1565 en_US
dc.identifier.source Journal of Raman Spectroscopy en_US
dc.owningcollname Interdisciplinary and Peer-Reviewed
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