ICOS and B7 costimulatory molecule expression identifies activated cellular subsets in rheumatoid arthritis
dc.contributor.author | Ruth, Jeffrey H. | en_US |
dc.contributor.author | Rottman, James B. | en_US |
dc.contributor.author | Kingsbury, Gillian A. | en_US |
dc.contributor.author | Coyle, Anthony J. | en_US |
dc.contributor.author | Haines, G. Kenneth III | en_US |
dc.contributor.author | Pope, Richard M. | en_US |
dc.contributor.author | Koch, Alisa E. | en_US |
dc.date.accessioned | 2007-09-20T18:35:12Z | |
dc.date.available | 2008-09-08T14:25:14Z | en_US |
dc.date.issued | 2007-05 | en_US |
dc.identifier.citation | Ruth, Jeffrey H.; Rottman, James B.; Kingsbury, Gillian A.; Coyle, Anthony J.; Haines, G. Kenneth; Pope, Richard M.; Koch, Alisa E. (2007)."ICOS and B7 costimulatory molecule expression identifies activated cellular subsets in rheumatoid arthritis." Cytometry Part A 71A(5): 317-326. <http://hdl.handle.net/2027.42/56032> | en_US |
dc.identifier.issn | 1552-4922 | en_US |
dc.identifier.issn | 1552-4930 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/56032 | |
dc.description.abstract | To better define important cell subsets expressing activation markers in rheumatoid arthritis (RA), we compared selective lymphocyte and monocyte B7H1, B7H2, B7RP.1, B7RP.2, and inducible costimulatory molecule (ICOS) expression from normal peripheral blood (NL PB), RA PB, and RA synovial fluid (SF) by multicolor flow cytometry and immunohistochemistry. RA SF memory lymphocytes expressed B7RP.1 and B7RP.2, suggesting that T-cells may function as antigen presenting cells (APCs) in RA joints. We found similar results for ICOS expression. RA SF CD14+ monocytes also expressed B7RP.1 (an ICOS ligand) and the homologous ligand B7RP.2, identifying monocytes as potential mediators of antigen processing and lymphocyte activation in RA. Furthermore, we found an increased population of RA SF CD14+ monocytes expressing B7H1 and B7H2. [The FACS analysis was supported by immunohistochemistry, showing intense lymphocyte and APC (macrophages with dendritic morphology) ICOS staining in RA synovial tissue (ST). Overall, these results define elevated populations of memoryT-lymphocytes expressing proinflammatory B7 molecules in RA SF that either stimulate T cells through ICOS (via ICOS ligands B7RP.1 and B7RP.2), or down-regulate RA ST T-lymphocytes through B7H1 and B7H2.] Therefore, in the same joint, there may exist positive and negative influences on the inflammatory response, and perhaps, the negative signals dominate as joint inflammation resolves. © 2007 International Society for Analytical Cytology | en_US |
dc.format.extent | 820249 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Cell & Developmental Biology | en_US |
dc.title | ICOS and B7 costimulatory molecule expression identifies activated cellular subsets in rheumatoid arthritis | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan 48109 ; Department of Internal Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois 60611 ; Research Assistant Professor of Internal Medicine, University of Michigan Medical School, Division of Rheumatology, 109 Zina Pitcher Place. 4380 BSRB, Box 2200, Ann Arbor, MI 48109-2200 | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan 48109 ; Department of Internal Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois 60611 ; Veteran's Administration, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationother | Inflammation | en_US |
dc.contributor.affiliationother | Experimental Medicine Divisions, Millenium Pharmaceuticals Inc., Cambridge, Massachusetts 02142 | en_US |
dc.contributor.affiliationother | Inflammation | en_US |
dc.contributor.affiliationother | Department of Pathology, Northwestern University Feinberg School of Medicine, Chicago, Illinois 60611 | en_US |
dc.contributor.affiliationother | Department of Internal Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois 60611 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/56032/1/20383_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/cyto.a.20383 | en_US |
dc.identifier.source | Cytometry Part A | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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