Validation of a two-parameter quantitative structure–activity relationship as a legitimate tool for rational re-design of horseradish peroxidase
dc.contributor.author | Colosi, Lisa M. | en_US |
dc.contributor.author | Huang, Qingguo | en_US |
dc.contributor.author | Weber, Walter J. | en_US |
dc.date.accessioned | 2007-09-20T18:57:21Z | |
dc.date.available | 2008-09-08T14:25:12Z | en_US |
dc.date.issued | 2007-09-01 | en_US |
dc.identifier.citation | Colosi, Lisa M.; Huang, Qingguo; Weber, Walter J. (2007)."Validation of a two-parameter quantitative structure–activity relationship as a legitimate tool for rational re-design of horseradish peroxidase." Biotechnology and Bioengineering 98(1): 295-299. <http://hdl.handle.net/2027.42/56109> | en_US |
dc.identifier.issn | 0006-3592 | en_US |
dc.identifier.issn | 1097-0290 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/56109 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=17657769&dopt=citation | en_US |
dc.description.abstract | Previously reported rates of reaction between six mutant strains of the enzyme horseradish peroxidase (HRP) and a test substrate, 2-methoyxpyhenol, were found to correlate with characteristic binding distances computed using molecular simulation. The correlation ( R 2 = 0.86) bears out a working hypothesis that, based on a quantitative structure–activity relationship (QSAR) we had previously developed for HRP, reductions in binding distances between the HRP enzyme and any selected substrate mediate increased enzyme reactivity towards that substrate. The results validate the use of QSAR as a quantitative means for formulating enzyme mutations designed to achieve enhanced HRP reactivity towards compounds of specific interest. Biotechnol. Bioeng. 2007; 98: 295–299. © 2007 Wiley Periodicals, Inc. | en_US |
dc.format.extent | 180068 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Biochemistry and Biotechnology | en_US |
dc.title | Validation of a two-parameter quantitative structure–activity relationship as a legitimate tool for rational re-design of horseradish peroxidase | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbsecondlevel | Mathematics | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Statistics and Numeric Data | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Social Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Civil & Environmental Engineering, University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Chemical Engineering, University of Michigan, 2200 Bonisteel Blvd, 4103 ERB I, Ann Arbor, Michigan 48109-2099; telephone: +1-734-763-1464; fax: +1-734-936-4391 ; Department of Crop and Soil Sciences, University of Georgia, Griffin, Georgia | en_US |
dc.contributor.affiliationum | Department of Chemical Engineering, University of Michigan, 2200 Bonisteel Blvd, 4103 ERB I, Ann Arbor, Michigan 48109-2099; telephone: +1-734-763-1464; fax: +1-734-936-4391 ; Department of Chemical Engineering, University of Michigan, 2200 Bonisteel Blvd, 4103 ERB I, Ann Arbor, Michigan 48109-2099; telephone: +1-734-763-1464; fax: +1-734-936-4391. | en_US |
dc.identifier.pmid | 17657769 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/56109/1/21419_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/bit.21419 | en_US |
dc.identifier.source | Biotechnology and Bioengineering | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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