Regulation of E-cadherin and Β-catenin by Ca 2+ in colon carcinoma is dependent on calcium-sensing receptor expression and function
dc.contributor.author | Bhagavathula, Narasimharao | en_US |
dc.contributor.author | Hanosh, Andrew W. | en_US |
dc.contributor.author | Nerusu, Kamalakar C. | en_US |
dc.contributor.author | Appelman, Henry D. | en_US |
dc.contributor.author | Chakrabarty, Subhas | en_US |
dc.contributor.author | Varani, James | en_US |
dc.date.accessioned | 2007-09-20T18:58:25Z | |
dc.date.available | 2008-11-05T15:05:43Z | en_US |
dc.date.issued | 2007-10-01 | en_US |
dc.identifier.citation | Bhagavathula, Narasimharao; Hanosh, Andrew W.; Nerusu, Kamalakar C.; Appelman, Henry; Chakrabarty, Subhas; Varani, James (2007)."Regulation of E-cadherin and Β-catenin by Ca 2+ in colon carcinoma is dependent on calcium-sensing receptor expression and function." International Journal of Cancer 121(7): 1455-1462. <http://hdl.handle.net/2027.42/56113> | en_US |
dc.identifier.issn | 0020-7136 | en_US |
dc.identifier.issn | 1097-0215 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/56113 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=17557293&dopt=citation | |
dc.description.abstract | An siRNA directed against the extracellular calcium-sensing receptor (CaSR) was used to down-regulate this protein in CBS colon carcinoma cells. In additional studies, we utilized a variant of the parental CBS line that demonstrates CaSR expression but does not upregulate this protein in response to extracellular Ca 2+ . In neither the siRNA-transfected cells nor the Ca 2+ -nonresponsive variant cells did inclusion of Ca 2+ in the culture medium inhibit proliferation or induce morphological alterations. Extracellular Ca 2+ also failed to induce E-cadherin production or a shift in Β-catenin from the cytoplasm to the cell membrane. In mock-transfected cells and in a Ca 2+ -responsive variant line derived from the same parental CBS cells, Ca 2+ treatment resulted in growth-reduction. This was accompanied by increased E-cadherin production and a shift in Β-catenin distribution from the cytoplasm to the cell membrane. Additionally, down-regulation of c-myc and cyclin D1 expression was observed in mock-transfected cells and in the Ca 2+ -responsive variant line (along with reduced T cell factor transcriptional activation). Neither c-myc nor cyclin D1 was significantly down-regulated in the siRNA-transfected cells or in the Ca 2+ -nonresponsive variant cells upon Ca 2+ stimulation. In histological sections of human colon carcinoma CaSR was significantly reduced as compared to the level in normal colonic crypt epithelial cells. Where CaSR expression was high, strong surface staining for E-cadherin and Β-catenin was observed. Where CaSR expression was reduced, Β-catenin surface expression was likewise reduced. © 2007 Wiley-Liss, Inc. | en_US |
dc.format.extent | 876244 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Cancer Research, Oncology and Pathology | en_US |
dc.title | Regulation of E-cadherin and Β-catenin by Ca 2+ in colon carcinoma is dependent on calcium-sensing receptor expression and function | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Oncology and Hematology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pathology, University of Michigan Medical School, Ann Arbor, MI | en_US |
dc.contributor.affiliationum | Department of Pathology, University of Michigan Medical School, Ann Arbor, MI | en_US |
dc.contributor.affiliationum | Department of Pathology, University of Michigan Medical School, Ann Arbor, MI | en_US |
dc.contributor.affiliationum | Department of Pathology, University of Michigan Medical School, Ann Arbor, MI | en_US |
dc.contributor.affiliationum | Department of Pathology, University of Michigan Medical School, Ann Arbor, MI ; Fax: +734-763-6476. ; Department of Pathology, The University of Michigan, 1301 Catherine Road/Box 0602, Ann Arbor, MI 48109, USA | en_US |
dc.contributor.affiliationother | Department of Medical Microbiology, Immunology and Cell Biology, Southern Illinois University Cancer Institute, Springfield, IL | en_US |
dc.identifier.pmid | 17557293 | |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/56113/1/22858_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/ijc.22858 | en_US |
dc.identifier.source | International Journal of Cancer | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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