Methylation profiling of mesothelioma using real-time methylation-specific PCR: A pilot study
dc.contributor.author | Pu, Robert T. | en_US |
dc.contributor.author | Sheng, Zong-Mei | en_US |
dc.contributor.author | Michael, Claire W. | en_US |
dc.contributor.author | Rhode, Michael G. | en_US |
dc.contributor.author | Clark, Douglas P. | en_US |
dc.contributor.author | O'Leary, Timothy J. | en_US |
dc.date.accessioned | 2007-09-20T19:16:58Z | |
dc.date.available | 2008-09-08T14:25:13Z | en_US |
dc.date.issued | 2007-08 | en_US |
dc.identifier.citation | Pu, Robert T.; Sheng, Zong-Mei; Michael, Claire W.; Rhode, Michael G.; Clark, Douglas P.; O'Leary, Timothy J. (2007)."Methylation profiling of mesothelioma using real-time methylation-specific PCR: A pilot study." Diagnostic Cytopathology 35(8): 498-502. <http://hdl.handle.net/2027.42/56179> | en_US |
dc.identifier.issn | 8755-1039 | en_US |
dc.identifier.issn | 1097-0339 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/56179 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=17636483&dopt=citation | en_US |
dc.description.abstract | We tested whether methylation profiles generated by real-time methylation-specific PCR (MSP) can be useful in differentiating benign, reactive mesothelial cell proliferation (RM) from malignant mesothelioma (MM). Forty-two of the 63 cases (67%) yielded informative results for RARΒ2, GPC3, CDKN2A (p16), TERT, and CCND2 (cyclinD2) gene methylation. DNA methylation of any gene was observed in much higher frequency in MM cases than RM cases (63% vs. 33%, P < 0.05). Individual gene methylation was higher in the MM than the RM cases for most of the genes; however, this was not statistically significant ( RARΒ 2 : 58% vs. 33%, P > 0.05; GPC3: 36% vs. 27%, P > 0.05; CDKN2A: 4% vs. 0%; TERT: 4% vs. 0%), while CCND2 methylation was not detected in any case. Although preliminary, we demonstrate that real-time MSP can be applied to archival specimens and gene methylation profiling may have potential to be a useful ancillary tool to help distinguish MM from RM . Diagn. Cytopathol. 2007;35:498–502. © 2007 Wiley-Liss, Inc. | en_US |
dc.format.extent | 141841 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Cancer Research, Oncology and Pathology | en_US |
dc.title | Methylation profiling of mesothelioma using real-time methylation-specific PCR: A pilot study | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Pathology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan ; Department of Pathology, The University of Michigan Medical School, 1500 E. Medical Center Drive, Room 2G332, Ann Arbor, Michigan, 48109 | en_US |
dc.contributor.affiliationum | Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationother | Department of Cellular Pathology and Genetics, Armed Forces Institute of Pathology, Rockville, Maryland | en_US |
dc.contributor.affiliationother | Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, Maryland | en_US |
dc.contributor.affiliationother | Department of Cellular Pathology and Genetics, Armed Forces Institute of Pathology, Rockville, Maryland ; Veterans Health Administration, Washington, District of Columbia | en_US |
dc.identifier.pmid | 17636483 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/56179/1/20692_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/dc.20692 | en_US |
dc.identifier.source | Diagnostic Cytopathology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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