Generation of mice with a conditional allele of the p120 Ras GTPase-activating protein
dc.contributor.author | Lapinski, Philip E. | en_US |
dc.contributor.author | Bauler, Timothy J. | en_US |
dc.contributor.author | Brown, Eric J. | en_US |
dc.contributor.author | Hughes, Elizabeth D. | en_US |
dc.contributor.author | Saunders, Thomas L. | en_US |
dc.contributor.author | King, Philip D. | en_US |
dc.date.accessioned | 2008-01-04T20:11:28Z | |
dc.date.available | 2009-01-07T20:01:15Z | en_US |
dc.date.issued | 2007-12 | en_US |
dc.identifier.citation | Lapinski, Philip E.; Bauler, Timothy J.; Brown, Eric J.; Hughes, Elizabeth D.; Saunders, Thomas L.; King, Philip D. (2007). "Generation of mice with a conditional allele of the p120 Ras GTPase-activating protein." genesis 45(12): 762-767. <http://hdl.handle.net/2027.42/57536> | en_US |
dc.identifier.issn | 1526-954X | en_US |
dc.identifier.issn | 1526-968X | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/57536 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=18064675&dopt=citation | en_US |
dc.description.abstract | p120 Ras GTPase-activating protein (RasGAP) encoded by the rasa1 gene in mice is a prototypical member of the RasGAP family of proteins involved in negative-regulation of the p21 Ras proto-oncogene. RasGAP has been implicated in signal transduction through a number of cell surface receptors. In humans, inactivating mutations in the coding region of the RASA1 gene cause capillary malformation arteriovenous malformation. In mice, generalized disruption of the rasa1 gene results in early embryonic lethality associated with defective vasculogenesis and increased apoptosis of neuronal cells. The early lethality in this mouse model precludes its use to further study the importance of RasGAP as a regulator of cell function. Therefore, to circumvent this problem, we have generated a conditional rasa1 knockout mouse. In this mouse, an exon that encodes a part of the RasGAP protein essential for catalytic activity has been flanked by loxP recognition sites. With the use of different constitutive and inducible Cre transgenic mouse lines, we show that deletion of this exon from the rasa1 locus results in effective loss of expression of catalytically-active RasGAP from a variety of adult tissues. The conditional rasa1 mouse will be useful for the analysis of the role of RasGAP in mature cell types. genesis 45:762–767, 2007. © 2007 Wiley-Liss, Inc. | en_US |
dc.format.extent | 221953 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Genetics | en_US |
dc.title | Generation of mice with a conditional allele of the p120 Ras GTPase-activating protein | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Genetics | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Transgenic Animal Model Core, University of Michigan Medical School, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Transgenic Animal Model Core, University of Michigan Medical School, Ann Arbor, Michigan 48109 ; Department of Internal Medicine, Division of Molecular Medicine and Genetics, University of Michigan Medical School, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan 48109 ; Department of Microbiology and Immunology, University of Michigan Medical School, 6606 Med. Sci. II, 1150 West Medical Center Drive, Ann Arbor, MI 48109-0620 | en_US |
dc.contributor.affiliationother | Department of Cancer Biology, Abramson Family Cancer Research Institute, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 | en_US |
dc.identifier.pmid | 18064675 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/57536/1/20354_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/dvg.20354 | en_US |
dc.identifier.source | genesis | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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