Now showing items 1-10 of 12
Synthesis of normal and variant human hypoxanthine-guanine phosphoribosyltransferase in Escherichia coli
(Elsevier, 1993-01-30)
Naturally occurring mutations in hypoxanthine-guanine phosphoribosyltransferase (HPRT) have been identified by amino acid sequencing, cDNA cloning, and direct nucleotide sequencing of PCR-amplified transcripts. To determine ...
Cell cycle progression is associated with distinct patterns of phosphorylation of Op18
(Elsevier, 1992-05-29)
Op18 is a highly conserved major cytosolic phosphoprotein which has been implicated in signal transduction in a wide variety of cell types. Freshly isolated peripheral blood lymphocytes (PBL) constitutively express low ...
Adenovirus binding to cultured synoviocytes triggers signaling through MAPK pathways and induces expression of cyclooxygenase-2
(John Wiley & Sons, Ltd., 2005-03)
Background Recombinant adenovirus can be administered in vivo to achieve transduction of a number of cell types including human synoviocytes. Immunogenicity of adenoviruses has limited their utility as vectors for gene ...
ELAM-1-dependent cell adhesion to vascular endothelium determined by a transfected human fucosyltransferase cDNA
(Elsevier, 1990-11-02)
Adhesion of circulating leukocytes to the vascular endothelium during inflammation is mediated in part by their interaction with the endothellal-leukocyte adhesion molecule ELAM-1. ELAM-1, a member of the LEC-CAM family ...
A 13 base pair deletion in exon 1 of HPRT Illinois forms a functional GUG initiation codon
(Springer-Verlag, 1994-03)
More than 50 mutations in the human hypoxanthine-guanine phosphoribosyltransferase (HPRT) locus have been described, yet only 2 alter the AUG initiation codon. One, variant HPRT 1151 , results in Lesch-Nyhan syndrome (LNS), ...
Use of receptors immobilized on microspheres to identify ligand binding sites in tissue sections: detection of lymph node ligands for L-Selectin
(Springer-Verlag; Springer-Verlag Berlin Heidelberg, 1997-01)
Particulate microspheres bearing immobilized probes can be used to identify ligands expressed by cells and require only brightfield microscopy for detection. There are distinct advantages to using microspheres to detect ...
Restituting intestinal epithelial cells exhibit increased transducibility by adenoviral vectors
(John Wiley & Sons, Ltd., 2006-12)
Background and aims While mature enterocytes are resistant to transduction by adenovirus type 5 (Ad5) vectors, undifferentiated cells are transduced much more efficiently. Our purpose was to study enterocyte transduction ...
Monokine-induced gene expression of a human endothelial cell-derived neutrophil chemotactic factor
(Elsevier, 1988-11-15)
Monokines have been increasingly recognized as communication signals that interact with both immune and non-immune cells during inflammation. Specifically, interleukin-1alpha (IL-lalpha), interleukin-1beta (IL-1beta) and ...
Direct plasmid mediated transfection of adult murine brain cells in vivo using cationic liposomes
(Elsevier, 1994-02-14)
Gene transfer to the central nervous system (CNS) is complicated by the anatomic and physiologic isolation of the brain. Direct injection techniques circumvent this, and allow delivery of transgenes to specific areas of ...
Cloning of cDNAs for human phosphoribosylpyrophosphate synthetases 1 and 2 and X chromosome localization of PRPS1 and PRPS2 genes
(Elsevier, 1990-11)
Cloned cDNAs representing the entire, homologous (80%) translated sequences of human phosphoribosylpyrophosphate synthetase (PRS) 1 and PRS 2 cDNAs were utilized as probes to localize the corresponding human PRPS1 and PRPS2 ...