Now showing items 1-10 of 17
Synthesis of normal and variant human hypoxanthine-guanine phosphoribosyltransferase in Escherichia coli
(Elsevier, 1993-01-30)
Naturally occurring mutations in hypoxanthine-guanine phosphoribosyltransferase (HPRT) have been identified by amino acid sequencing, cDNA cloning, and direct nucleotide sequencing of PCR-amplified transcripts. To determine ...
Effect of an inhibitor of DNA methylation on T cells. II. 5-azacytidine induces self-reactivity in antigen-specific T4+ cells
(Elsevier, 1986-12)
During T-cell maturation, thymocytes interact with thymic stromal major histocompatibility complex (MHC) determinants and thymic hormones, and proliferate, apparently in response to MHC gene products, in the absence of ...
The acute inflammatory reaction
(Elsevier, 1989-10)
A major theme in current developments in this field has been the realization that the target tissue, in particular the endothelium, has an active role in initiating and participating in inflammatory reactions and is not a ...
Evidence that macrophages are programmed to die after activating autologous, cloned, antigen-specific, CD4 + T cells
(WILEY-VCH Verlag GmbH, 1993-07)
The bone marrow produces large numbers of monocytes daily, but the mechanisms balancing production and elimination are unknown. In this report we demonstrate that macrophages (Mφ) undergo apoptosis after activating autologous ...
Adenovirus binding to cultured synoviocytes triggers signaling through MAPK pathways and induces expression of cyclooxygenase-2
(John Wiley & Sons, Ltd., 2005-03)
Background Recombinant adenovirus can be administered in vivo to achieve transduction of a number of cell types including human synoviocytes. Immunogenicity of adenoviruses has limited their utility as vectors for gene ...
Decay-accelerating factor is a component of subendothelial extracellular matrix in vitro , and is augmented by activation of endothelial protein kinase C
(WILEY-VCH Verlag GmbH, 1998-03)
The vasculature is protected from complement activation by regulatory molecules expressed on endothelial cells. However, complement fixation also occurs on subendothelial extracellular matrix (ECM) in vitro, and is ...
Fas ligation triggers apoptosis in macrophages but not endothelial cells
(WILEY-VCH Verlag GmbH, 1994-11)
The reticuloendothelial system includes macrophages and endothelial cells. These cells are produced and destroyed in vivo with a precision that implies the existence of homeostatic mechanisms. The stimuli for endothelial ...
Activation of human T cell clones through the UM4D4/CDw60 surface antigen
(Elsevier, 1990-07)
UM4D4 is a recently defined antigen that is expressed on ~25% of peripheral blood T cells, but on the majority of T cells in inflammatory synovial fluid. Anti-UM4D4 activates peripheral blood T cells in the presence of ...
A 13 base pair deletion in exon 1 of HPRT Illinois forms a functional GUG initiation codon
(Springer-Verlag, 1994-03)
More than 50 mutations in the human hypoxanthine-guanine phosphoribosyltransferase (HPRT) locus have been described, yet only 2 alter the AUG initiation codon. One, variant HPRT 1151 , results in Lesch-Nyhan syndrome (LNS), ...
Restituting intestinal epithelial cells exhibit increased transducibility by adenoviral vectors
(John Wiley & Sons, Ltd., 2006-12)
Background and aims While mature enterocytes are resistant to transduction by adenovirus type 5 (Ad5) vectors, undifferentiated cells are transduced much more efficiently. Our purpose was to study enterocyte transduction ...