Now showing items 1-10 of 16
Synthesis of normal and variant human hypoxanthine-guanine phosphoribosyltransferase in Escherichia coli
(Elsevier, 1993-01-30)
Naturally occurring mutations in hypoxanthine-guanine phosphoribosyltransferase (HPRT) have been identified by amino acid sequencing, cDNA cloning, and direct nucleotide sequencing of PCR-amplified transcripts. To determine ...
Evidence that macrophages are programmed to die after activating autologous, cloned, antigen-specific, CD4 + T cells
(WILEY-VCH Verlag GmbH, 1993-07)
The bone marrow produces large numbers of monocytes daily, but the mechanisms balancing production and elimination are unknown. In this report we demonstrate that macrophages (Mφ) undergo apoptosis after activating autologous ...
CD4+ cells treated with DNA methylation inhibitors induce autologous B cell differentiation
(Elsevier, 1990-06)
The DNA methylation inhibitor 5-azacytidine induces autoreactivity in cloned CD4+ T cells, but the functional consequences of this response are unknown. We now report that CD4+ T cells treated with 5-azacytidine respond ...
Cell cycle progression is associated with distinct patterns of phosphorylation of Op18
(Elsevier, 1992-05-29)
Op18 is a highly conserved major cytosolic phosphoprotein which has been implicated in signal transduction in a wide variety of cell types. Freshly isolated peripheral blood lymphocytes (PBL) constitutively express low ...
Decay-accelerating factor is a component of subendothelial extracellular matrix in vitro , and is augmented by activation of endothelial protein kinase C
(WILEY-VCH Verlag GmbH, 1998-03)
The vasculature is protected from complement activation by regulatory molecules expressed on endothelial cells. However, complement fixation also occurs on subendothelial extracellular matrix (ECM) in vitro, and is ...
ELAM-1-dependent cell adhesion to vascular endothelium determined by a transfected human fucosyltransferase cDNA
(Elsevier, 1990-11-02)
Adhesion of circulating leukocytes to the vascular endothelium during inflammation is mediated in part by their interaction with the endothellal-leukocyte adhesion molecule ELAM-1. ELAM-1, a member of the LEC-CAM family ...
Fas ligation triggers apoptosis in macrophages but not endothelial cells
(WILEY-VCH Verlag GmbH, 1994-11)
The reticuloendothelial system includes macrophages and endothelial cells. These cells are produced and destroyed in vivo with a precision that implies the existence of homeostatic mechanisms. The stimuli for endothelial ...
Activation of human T cell clones through the UM4D4/CDw60 surface antigen
(Elsevier, 1990-07)
UM4D4 is a recently defined antigen that is expressed on ~25% of peripheral blood T cells, but on the majority of T cells in inflammatory synovial fluid. Anti-UM4D4 activates peripheral blood T cells in the presence of ...
A 13 base pair deletion in exon 1 of HPRT Illinois forms a functional GUG initiation codon
(Springer-Verlag, 1994-03)
More than 50 mutations in the human hypoxanthine-guanine phosphoribosyltransferase (HPRT) locus have been described, yet only 2 alter the AUG initiation codon. One, variant HPRT 1151 , results in Lesch-Nyhan syndrome (LNS), ...
Use of receptors immobilized on microspheres to identify ligand binding sites in tissue sections: detection of lymph node ligands for L-Selectin
(Springer-Verlag; Springer-Verlag Berlin Heidelberg, 1997-01)
Particulate microspheres bearing immobilized probes can be used to identify ligands expressed by cells and require only brightfield microscopy for detection. There are distinct advantages to using microspheres to detect ...