Reishi immuno-modulation protein induces interleukin-2 expression via protein kinase-dependent signaling pathways within human T cells Hsien-Yeh Hsu and Kuo-Feng Hua contributed equally to this study.
dc.contributor.author | Hsu, Hsien-Yeh | en_US |
dc.contributor.author | Hua, Kuo-Feng | en_US |
dc.contributor.author | Wu, Wei-Chi | en_US |
dc.contributor.author | Hsu, Jason | en_US |
dc.contributor.author | Weng, Shih-Ting | en_US |
dc.contributor.author | Lin, Tsai-Leng | en_US |
dc.contributor.author | Liu, Chun-Yi | en_US |
dc.contributor.author | Hseu, Ruey-Shyang | en_US |
dc.contributor.author | Huang, Ching-Tsan | en_US |
dc.date.accessioned | 2008-02-04T19:16:27Z | |
dc.date.available | 2009-04-09T15:01:14Z | en_US |
dc.date.issued | 2008-04 | en_US |
dc.identifier.citation | Hsu, Hsien-Yeh; Hua, Kuo-Feng; Wu, Wei-Chi; Hsu, Jason; Weng, Shih-Ting; Lin, Tsai-Leng; Liu, Chun-Yi; Hseu, Ruey-Shyang; Huang, Ching-Tsan (2008). "Reishi immuno-modulation protein induces interleukin-2 expression via protein kinase-dependent signaling pathways within human T cells Hsien-Yeh Hsu and Kuo-Feng Hua contributed equally to this study. ." Journal of Cellular Physiology 215(1): 15-26. <http://hdl.handle.net/2027.42/57902> | en_US |
dc.identifier.issn | 0021-9541 | en_US |
dc.identifier.issn | 1097-4652 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/57902 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=18189229&dopt=citation | |
dc.description.abstract | Ganoderma lucidum , a medicinal fungus is thought to possess and enhance a variety of human immune functions. An immuno-modulatory protein, Ling Zhi-8 (LZ-8) isolated from G. lucidum exhibited potent mitogenic effects upon human peripheral blood lymphocytes (PBL). However, LZ-8-mediated signal transduction in the regulation of interleukin-2 (IL-2) gene expression within human T cells is largely unknown. Here we cloned the LZ-8 gene of G. lucidum , and expressed the recombinant LZ-8 protein (rLZ-8) by means of a yeast Pichia pastoris protein expression system. We found that rLZ-8 induces IL-2 gene expression via the Src-family protein tyrosine kinase (PTK), via reactive oxygen species (ROS), and differential protein kinase-dependent pathways within human primary T cells and cultured Jurkat T cells. In essence, we have established the nature of the rLZ-8-mediated signal-transduction pathways, such as PTK/protein kinase C (PKC)/ROS, PTK/PLC/PKCalpha/ERK1/2, and PTK/PLC/PKCalpha/p38 pathways in the regulation of IL-2 gene expression within human T cells. Our current results of analyzing rLZ-8-mediated signal transduction in T cells might provide a potential application for rLZ-8 as a pharmacological immune-modulating agent. J. Cell. Physiol. 215: 15–26, 2008. © 2008 Wiley-Liss, Inc. | en_US |
dc.format.extent | 464799 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Cell & Developmental Biology | en_US |
dc.title | Reishi immuno-modulation protein induces interleukin-2 expression via protein kinase-dependent signaling pathways within human T cells Hsien-Yeh Hsu and Kuo-Feng Hua contributed equally to this study. | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Kinesiology and Sports | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Stephen M. Ross School of Business, University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationother | Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei, Taiwan ; Institute of Biophotonics Engineering, National Yang-Ming University, Taipei, Taiwan ; Department of Education and Research, Taipei City Hospital, Taiwan ; Institute of Biotechnology in Medicine, National Yang-Ming University, 155 Li-Nong Street, Shih-Pai, Taipei, Taiwan. | en_US |
dc.contributor.affiliationother | Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei, Taiwan ; Institute of Biophotonics Engineering, National Yang-Ming University, Taipei, Taiwan | en_US |
dc.contributor.affiliationother | Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei, Taiwan | en_US |
dc.contributor.affiliationother | Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei, Taiwan | en_US |
dc.contributor.affiliationother | Department of Biochemical Science and Technology, Institute of Microbiology and Biochemistry, National Taiwan University, Taipei, Taiwan | en_US |
dc.contributor.affiliationother | Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei, Taiwan | en_US |
dc.contributor.affiliationother | Department of Biochemical Science and Technology, Institute of Microbiology and Biochemistry, National Taiwan University, Taipei, Taiwan ; Department of Biochemical Science and Technology, Institute of Microbiology and Biochemistry, National Taiwan University, Taipei, Taiwan. | en_US |
dc.contributor.affiliationother | Department of Biochemical Science and Technology, Institute of Microbiology and Biochemistry, National Taiwan University, Taipei, Taiwan ; Department of Biochemical Science and Technology, Institute of Microbiology and Biochemistry, National Taiwan University, Taipei, Taiwan. | en_US |
dc.identifier.pmid | 18189229 | |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/57902/1/21144_ftp.pdf | |
dc.identifier.doi | http://dx.doi.org/10.1002/jcp.21144 | en_US |
dc.identifier.source | Journal of Cellular Physiology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.