CE of poly(amidoamine) succinamic acid dendrimers using a poly(vinyl alcohol)-coated capillary
dc.contributor.author | Desai, Ankur M. | en_US |
dc.contributor.author | Shi, Xiangyang | en_US |
dc.contributor.author | Baker, James R. Jr. | en_US |
dc.date.accessioned | 2008-02-04T19:18:23Z | |
dc.date.available | 2009-01-07T20:01:16Z | en_US |
dc.date.issued | 2008-01 | en_US |
dc.identifier.citation | Desai, Ankur; Shi, Xiangyang; Baker, James R. (2008). "CE of poly(amidoamine) succinamic acid dendrimers using a poly(vinyl alcohol)-coated capillary." Electrophoresis 29(2): 510-515. <http://hdl.handle.net/2027.42/57911> | en_US |
dc.identifier.issn | 0173-0835 | en_US |
dc.identifier.issn | 1522-2683 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/57911 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=18080252&dopt=citation | en_US |
dc.description.abstract | Various generations (G1–G8) of negatively charged poly(amidoamine) (PAMAM) succinamic acid dendrimers (PAMAM-SAH) were analyzed by CE using a poly(vinyl alcohol)-coated capillary. Due to its excellent stability and osmotic flow-shielding effect, highly reproducible migration times were achieved for all generations of dendrimer ( e.g ., RSD for the migration times of G5 dendrimer was 0.6%). We also observed a reverse trend in migration times for the PAMAM-SAH dendrimers ( i.e ., higher generations migrated faster than lower generation dendrimers) compared to amine-terminated PAMAM dendrimers reported in the literature. This reversal in migration times was attributed to the difference in counterion binding around these negatively charged dendrimers. This reverse trend allowed a generational separation for lower generation (G1–G3) dendrimers. However, a sufficient resolution for the migration peaks of higher generations (G4–G5) in a mixture could not be achieved. This could be due to their nearly identical charge/mass ratio and dense molecular conformations. In addition, we show that dye-functionalized PAMAM-SAH dendrimers can also be analyzed with high reproducibility using this method. | en_US |
dc.format.extent | 326769 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | WILEY-VCH Verlag | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Biochemistry and Biotechnology | en_US |
dc.title | CE of poly(amidoamine) succinamic acid dendrimers using a poly(vinyl alcohol)-coated capillary | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Michigan Nanotechnology for Medicine and Biological Sciences, University of Michigan, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Michigan Nanotechnology for Medicine and Biological Sciences, University of Michigan, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Michigan Nanotechnology for Medicine and Biological Sciences, University of Michigan, Ann Arbor, MI, USA ; Michigan Nanotechnology for Medicine and Biological Sciences, University of Michigan, Ann Arbor, MI 48109, USA Fax: +1-734-936-2990 | en_US |
dc.identifier.pmid | 18080252 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/57911/1/510_ftp.pdf | |
dc.identifier.doi | http://dx.doi.org/10.1002/elps.200700454 | en_US |
dc.identifier.source | Electrophoresis | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.