Comparison of PAX-2, RCC antigen, and antiphosphorylated H2AX antibody (Γ-H2AX) in diagnosing metastatic renal cell carcinoma by fine-needle aspiration
dc.contributor.author | Wasco, Matthew J. | en_US |
dc.contributor.author | Pu, Robert T. | en_US |
dc.date.accessioned | 2008-08-04T15:13:24Z | |
dc.date.available | 2009-08-12T18:32:18Z | en_US |
dc.date.issued | 2008-08 | en_US |
dc.identifier.citation | Wasco, Matthew J.; Pu, Robert T. (2008). "Comparison of PAX-2, RCC antigen, and antiphosphorylated H2AX antibody (Γ-H2AX) in diagnosing metastatic renal cell carcinoma by fine-needle aspiration." Diagnostic Cytopathology 36(8): 568-573. <http://hdl.handle.net/2027.42/60448> | en_US |
dc.identifier.issn | 8755-1039 | en_US |
dc.identifier.issn | 1097-0339 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/60448 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=18618717&dopt=citation | en_US |
dc.description.abstract | Diagnosing metastatic renal cell carcinoma (RCC) by fine-needle aspiration (FNA) can be challenging. Existing antibodies supporting a diagnosis of RCC, including CD10 and RCC-Ma, have problems with specificity and interpretation. In this report, we evaluate the use of two newer immunostains, PAX-2 and Γ-H2AX, which to our knowledge have not been studied in FNA material, in the diagnosis of metastatic RCC and in comparison with RCC-Ma. 29 cases of metastatic RCC were identified as well as a TMA of an additional 30 RCC cases. In the case cohort, RCC-Ma in a membranous pattern of staining identified 15/27 (56%) metastatic RCC, although interpretation was made difficult in many cases due to focality of staining and non-specific cytoplasmic staining. PAX-2 stained 23/29 (79%) of tumors in a nuclear stain, most strongly. Gamma-H2AX stained 19/26 (73%) of metastatic RCC strongly in a nuclear stain. In the TMA, strong, diffuse nuclear staining with Γ-H2AX was present in 22/30 RCC (73%). If weak staining was also included as positive, 26/30 (87%) were positive. PAX-2 stained RCC TMA with a lower percentage at 56%, including weaker staining intensity. Both PAX-2 and Γ-H2AX demonstrated patchy staining of normal renal tubules, PAX-2 to a greater extent. Both PAX-2 and Γ-H2AX are sensitive markers for the diagnosis of metastatic RCC, with improved ease of interpretation when compared with RCC-Ma. A combination of all 3 markers identified 87% of cases, and failure to stain for both PAX-2 and Γ-H2AX suggests against, but does not disprove, a diagnosis of RCC. Diagn. Cytopathol. 2008; 36: 568–573. © 2008 Wiley-Liss, Inc. | en_US |
dc.format.extent | 388156 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Cancer Research, Oncology and Pathology | en_US |
dc.title | Comparison of PAX-2, RCC antigen, and antiphosphorylated H2AX antibody (Γ-H2AX) in diagnosing metastatic renal cell carcinoma by fine-needle aspiration | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Pathology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | The University of Michigan Medical School, Department of Pathology, Ann Arbor, Michigan ; The University of Michigan Medical School, Department of Pathology, 1301 Catherine, M4211 MSI Ann Arbor, MI 48109-0602 | en_US |
dc.contributor.affiliationum | The University of Michigan Medical School, Department of Pathology, Ann Arbor, Michigan | en_US |
dc.identifier.pmid | 18618717 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/60448/1/20839_ftp.pdf | |
dc.identifier.doi | http://dx.doi.org/10.1002/dc.20839 | en_US |
dc.identifier.source | Diagnostic Cytopathology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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