Novel surface expression of reticulocalbin 1 on bone endothelial cells and human prostate cancer cells is regulated by TNF-Α
dc.contributor.author | Cooper, Carlton R. | en_US |
dc.contributor.author | Graves, Bianca | en_US |
dc.contributor.author | Pruitt, Freddie | en_US |
dc.contributor.author | Chaib, Hassan | en_US |
dc.contributor.author | Lynch, Jill E. | en_US |
dc.contributor.author | Cox, Andrew Koemeter | en_US |
dc.contributor.author | Sequeria, Linda | en_US |
dc.contributor.author | van Golen, Kenneth L. | en_US |
dc.contributor.author | Evans, Angelo | en_US |
dc.contributor.author | Czymmek, Kirk | en_US |
dc.contributor.author | Bullard, Rebecca S. | en_US |
dc.contributor.author | Donald, Carlton D. | en_US |
dc.contributor.author | Sol-Church, Katia | en_US |
dc.contributor.author | Gendernalik, James D. | en_US |
dc.contributor.author | Weksler, Babette | en_US |
dc.contributor.author | Farach-Carson, Mary C. | en_US |
dc.contributor.author | Macoska, Jill A. | en_US |
dc.contributor.author | Sikes, Robert A. | en_US |
dc.contributor.author | Pienta, Kenneth J. | en_US |
dc.date.accessioned | 2008-08-04T15:14:40Z | |
dc.date.available | 2009-09-02T14:40:29Z | en_US |
dc.date.issued | 2008-08-15 | en_US |
dc.identifier.citation | Cooper, Carlton R.; Graves, Bianca; Pruitt, Freddie; Chaib, Hassan; Lynch, Jill E.; Cox, Andrew Koemeter; Sequeria, Linda; van Golen, Kenneth L.; Evans, Angelo; Czymmek, Kirk; Bullard, Rebecca S.; Donald, Carlton D.; Sol-Church, Katia; Gendernalik, James D.; Weksler, Babette; Farach-Carson, Mary C.; Macoska, Jill A.; Sikes, Robert A.; Pienta, Kenneth J. (2008). "Novel surface expression of reticulocalbin 1 on bone endothelial cells and human prostate cancer cells is regulated by TNF-Α." Journal of Cellular Biochemistry 104(6): 2298-2309. <http://hdl.handle.net/2027.42/60470> | en_US |
dc.identifier.issn | 0730-2312 | en_US |
dc.identifier.issn | 1097-4644 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/60470 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=18561328&dopt=citation | en_US |
dc.description.abstract | An unbiased cDNA expression phage library derived from bone-marrow endothelial cells was used to identify novel surface adhesion molecules that might participate in metastasis. Herein we report that reticulocalbin 1 (RCN1) is a cell surface-associated protein on both endothelial (EC) and prostate cancer (PCa) cell lines. RCN1 is an H/KDEL protein with six EF-hand, calcium-binding motifs, found in the endoplasmic reticulum. Our data indicate that RCN1 also is expressed on the cell surface of several endothelial cell lines, including human dermal microvascular endothelial cells (HDMVECs), bone marrow endothelial cells (BMEC), and transformed human bone marrow endothelial cells (TrHBMEC). While RCN1 protein levels were highest in lysates from HDMVEC, this difference was not statistically significant compared BMEC and TrHBMEC. Given preferential adhesion of PCa to bone-marrow EC, these data suggest that RCN1 is unlikely to account for the preferential metastasis of PCa to bone. In addition, there was not a statistically significant difference in total RCN1 protein expression among the PCa cell lines. RCN1 also was expressed on the surface of several PCa cell lines, including those of the LNCaP human PCa progression model and the highly metastatic PC-3 cell line. Interestingly, RCN1 expression on the cell surface was upregulated by tumor necrosis factor alpha treatment of bone-marrow endothelial cells. Taken together, we show cell surface localization of RCN1 that has not been described previously for either PCa or BMEC and that the surface expression on BMEC is regulated by pro-inflammatory TNF-Α. J. Cell. Biochem. 104: 2298–2309, 2008. © 2008 Wiley-Liss, Inc. | en_US |
dc.format.extent | 385406 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Cell & Developmental Biology | en_US |
dc.title | Novel surface expression of reticulocalbin 1 on bone endothelial cells and human prostate cancer cells is regulated by TNF-Α | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Genetics | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Departments of Internal Medicine and/or Urology, Comprehensive Cancer Center, University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Section of Hematology/Oncology, Departments of Internal Medicine and Urology, Comprehensive Cancer Center, University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Departments of Internal Medicine and/or Urology, Comprehensive Cancer Center, University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Section of Hematology/Oncology, Departments of Internal Medicine and Urology, Comprehensive Cancer Center, University of Michigan, Ann Arbor, Michigan ; Departments of Internal Medicine and/or Urology, Comprehensive Cancer Center, University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationother | Center for Translational Cancer Research and Department of Biological Sciences, University of Delaware, Newark, Delaware ; Department of Biological Sciences, University of Delaware, 324 Wolf Hall, Newark, DE 19716. | en_US |
dc.contributor.affiliationother | Center for Translational Cancer Research and Department of Biological Sciences, University of Delaware, Newark, Delaware | en_US |
dc.contributor.affiliationother | Center for Translational Cancer Research and Department of Biological Sciences, University of Delaware, Newark, Delaware | en_US |
dc.contributor.affiliationother | Center for Translational Cancer Research and Department of Biological Sciences, University of Delaware, Newark, Delaware | en_US |
dc.contributor.affiliationother | Center for Translational Cancer Research and Department of Biological Sciences, University of Delaware, Newark, Delaware | en_US |
dc.contributor.affiliationother | Center for Translational Cancer Research and Department of Biological Sciences, University of Delaware, Newark, Delaware | en_US |
dc.contributor.affiliationother | Center for Translational Cancer Research and Department of Biological Sciences, University of Delaware, Newark, Delaware | en_US |
dc.contributor.affiliationother | Center for Translational Cancer Research and Department of Biological Sciences, University of Delaware, Newark, Delaware | en_US |
dc.contributor.affiliationother | Center for Translational Cancer Research and Department of Biological Sciences, University of Delaware, Newark, Delaware ; Delaware Biotechnology Institute, Department of Biological Sciences, University of Delaware, Newark, Delaware | en_US |
dc.contributor.affiliationother | Department of Pathology and Lab Medicine, Medical University of Charleston, Charleston, South Carolina | en_US |
dc.contributor.affiliationother | Department of Pathology and Lab Medicine, Medical University of Charleston, Charleston, South Carolina | en_US |
dc.contributor.affiliationother | Biomedical Research Department, Nemours Children Clinic, Wilmington, Delaware | en_US |
dc.contributor.affiliationother | Department of Medicine, Weill Medical College of Cornell University, New York, New York | en_US |
dc.contributor.affiliationother | Center for Translational Cancer Research and Department of Biological Sciences, University of Delaware, Newark, Delaware | en_US |
dc.contributor.affiliationother | Center for Translational Cancer Research and Department of Biological Sciences, University of Delaware, Newark, Delaware | en_US |
dc.identifier.pmid | 18561328 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/60470/1/21785_ftp.pdf | |
dc.identifier.doi | http://dx.doi.org/10.1002/jcb.21785 | en_US |
dc.identifier.source | Journal of Cellular Biochemistry | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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