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Novel surface expression of reticulocalbin 1 on bone endothelial cells and human prostate cancer cells is regulated by TNF-Α

dc.contributor.authorCooper, Carlton R.en_US
dc.contributor.authorGraves, Biancaen_US
dc.contributor.authorPruitt, Freddieen_US
dc.contributor.authorChaib, Hassanen_US
dc.contributor.authorLynch, Jill E.en_US
dc.contributor.authorCox, Andrew Koemeteren_US
dc.contributor.authorSequeria, Lindaen_US
dc.contributor.authorvan Golen, Kenneth L.en_US
dc.contributor.authorEvans, Angeloen_US
dc.contributor.authorCzymmek, Kirken_US
dc.contributor.authorBullard, Rebecca S.en_US
dc.contributor.authorDonald, Carlton D.en_US
dc.contributor.authorSol-Church, Katiaen_US
dc.contributor.authorGendernalik, James D.en_US
dc.contributor.authorWeksler, Babetteen_US
dc.contributor.authorFarach-Carson, Mary C.en_US
dc.contributor.authorMacoska, Jill A.en_US
dc.contributor.authorSikes, Robert A.en_US
dc.contributor.authorPienta, Kenneth J.en_US
dc.date.accessioned2008-08-04T15:14:40Z
dc.date.available2009-09-02T14:40:29Zen_US
dc.date.issued2008-08-15en_US
dc.identifier.citationCooper, Carlton R.; Graves, Bianca; Pruitt, Freddie; Chaib, Hassan; Lynch, Jill E.; Cox, Andrew Koemeter; Sequeria, Linda; van Golen, Kenneth L.; Evans, Angelo; Czymmek, Kirk; Bullard, Rebecca S.; Donald, Carlton D.; Sol-Church, Katia; Gendernalik, James D.; Weksler, Babette; Farach-Carson, Mary C.; Macoska, Jill A.; Sikes, Robert A.; Pienta, Kenneth J. (2008). "Novel surface expression of reticulocalbin 1 on bone endothelial cells and human prostate cancer cells is regulated by TNF-Α." Journal of Cellular Biochemistry 104(6): 2298-2309. <http://hdl.handle.net/2027.42/60470>en_US
dc.identifier.issn0730-2312en_US
dc.identifier.issn1097-4644en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/60470
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=18561328&dopt=citationen_US
dc.description.abstractAn unbiased cDNA expression phage library derived from bone-marrow endothelial cells was used to identify novel surface adhesion molecules that might participate in metastasis. Herein we report that reticulocalbin 1 (RCN1) is a cell surface-associated protein on both endothelial (EC) and prostate cancer (PCa) cell lines. RCN1 is an H/KDEL protein with six EF-hand, calcium-binding motifs, found in the endoplasmic reticulum. Our data indicate that RCN1 also is expressed on the cell surface of several endothelial cell lines, including human dermal microvascular endothelial cells (HDMVECs), bone marrow endothelial cells (BMEC), and transformed human bone marrow endothelial cells (TrHBMEC). While RCN1 protein levels were highest in lysates from HDMVEC, this difference was not statistically significant compared BMEC and TrHBMEC. Given preferential adhesion of PCa to bone-marrow EC, these data suggest that RCN1 is unlikely to account for the preferential metastasis of PCa to bone. In addition, there was not a statistically significant difference in total RCN1 protein expression among the PCa cell lines. RCN1 also was expressed on the surface of several PCa cell lines, including those of the LNCaP human PCa progression model and the highly metastatic PC-3 cell line. Interestingly, RCN1 expression on the cell surface was upregulated by tumor necrosis factor alpha treatment of bone-marrow endothelial cells. Taken together, we show cell surface localization of RCN1 that has not been described previously for either PCa or BMEC and that the surface expression on BMEC is regulated by pro-inflammatory TNF-Α. J. Cell. Biochem. 104: 2298–2309, 2008. © 2008 Wiley-Liss, Inc.en_US
dc.format.extent385406 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.publisherWiley Subscription Services, Inc., A Wiley Companyen_US
dc.subject.otherLife and Medical Sciencesen_US
dc.subject.otherCell & Developmental Biologyen_US
dc.titleNovel surface expression of reticulocalbin 1 on bone endothelial cells and human prostate cancer cells is regulated by TNF-Αen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelGeneticsen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartments of Internal Medicine and/or Urology, Comprehensive Cancer Center, University of Michigan, Ann Arbor, Michiganen_US
dc.contributor.affiliationumSection of Hematology/Oncology, Departments of Internal Medicine and Urology, Comprehensive Cancer Center, University of Michigan, Ann Arbor, Michiganen_US
dc.contributor.affiliationumDepartments of Internal Medicine and/or Urology, Comprehensive Cancer Center, University of Michigan, Ann Arbor, Michiganen_US
dc.contributor.affiliationumSection of Hematology/Oncology, Departments of Internal Medicine and Urology, Comprehensive Cancer Center, University of Michigan, Ann Arbor, Michigan ; Departments of Internal Medicine and/or Urology, Comprehensive Cancer Center, University of Michigan, Ann Arbor, Michiganen_US
dc.contributor.affiliationotherCenter for Translational Cancer Research and Department of Biological Sciences, University of Delaware, Newark, Delaware ; Department of Biological Sciences, University of Delaware, 324 Wolf Hall, Newark, DE 19716.en_US
dc.contributor.affiliationotherCenter for Translational Cancer Research and Department of Biological Sciences, University of Delaware, Newark, Delawareen_US
dc.contributor.affiliationotherCenter for Translational Cancer Research and Department of Biological Sciences, University of Delaware, Newark, Delawareen_US
dc.contributor.affiliationotherCenter for Translational Cancer Research and Department of Biological Sciences, University of Delaware, Newark, Delawareen_US
dc.contributor.affiliationotherCenter for Translational Cancer Research and Department of Biological Sciences, University of Delaware, Newark, Delawareen_US
dc.contributor.affiliationotherCenter for Translational Cancer Research and Department of Biological Sciences, University of Delaware, Newark, Delawareen_US
dc.contributor.affiliationotherCenter for Translational Cancer Research and Department of Biological Sciences, University of Delaware, Newark, Delawareen_US
dc.contributor.affiliationotherCenter for Translational Cancer Research and Department of Biological Sciences, University of Delaware, Newark, Delawareen_US
dc.contributor.affiliationotherCenter for Translational Cancer Research and Department of Biological Sciences, University of Delaware, Newark, Delaware ; Delaware Biotechnology Institute, Department of Biological Sciences, University of Delaware, Newark, Delawareen_US
dc.contributor.affiliationotherDepartment of Pathology and Lab Medicine, Medical University of Charleston, Charleston, South Carolinaen_US
dc.contributor.affiliationotherDepartment of Pathology and Lab Medicine, Medical University of Charleston, Charleston, South Carolinaen_US
dc.contributor.affiliationotherBiomedical Research Department, Nemours Children Clinic, Wilmington, Delawareen_US
dc.contributor.affiliationotherDepartment of Medicine, Weill Medical College of Cornell University, New York, New Yorken_US
dc.contributor.affiliationotherCenter for Translational Cancer Research and Department of Biological Sciences, University of Delaware, Newark, Delawareen_US
dc.contributor.affiliationotherCenter for Translational Cancer Research and Department of Biological Sciences, University of Delaware, Newark, Delawareen_US
dc.identifier.pmid18561328en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/60470/1/21785_ftp.pdf
dc.identifier.doihttp://dx.doi.org/10.1002/jcb.21785en_US
dc.identifier.sourceJournal of Cellular Biochemistryen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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