Interpretation of positive transcription-mediated amplification test results from polymerase chain reaction–negative samples obtained after treatment of chronic hepatitis C
dc.contributor.author | Morishima, Chihiro | en_US |
dc.contributor.author | Morgan, Timothy R. | en_US |
dc.contributor.author | Everhart, James E. | en_US |
dc.contributor.author | Wright, Elizabeth C. | en_US |
dc.contributor.author | Apodaca, Minjun C. | en_US |
dc.contributor.author | Gretch, David R. | en_US |
dc.contributor.author | Shiffman, Mitchell L. | en_US |
dc.contributor.author | Everson, Gregory T. | en_US |
dc.contributor.author | Lindsay, Karen L. | en_US |
dc.contributor.author | Lee, William M. | en_US |
dc.contributor.author | Lok, Anna Suk-Fong | en_US |
dc.contributor.author | Dienstag, Jules L. | en_US |
dc.contributor.author | Ghany, Marc G. | en_US |
dc.contributor.author | Curto, Teresa M. | en_US |
dc.date.accessioned | 2008-12-01T21:00:27Z | |
dc.date.available | 2010-01-05T16:59:13Z | en_US |
dc.date.issued | 2008-11 | en_US |
dc.identifier.citation | Morishima, Chihiro; Morgan, Timothy R.; Everhart, James E.; Wright, Elizabeth C.; Apodaca, Minjun C.; Gretch, David R.; Shiffman, Mitchell L.; Everson, Gregory T.; Lindsay, Karen L.; Lee, William M.; Lok, Anna S. F.; Dienstag, Jules L.; Ghany, Marc G.; Curto, Teresa M. (2008). "Interpretation of positive transcription-mediated amplification test results from polymerase chain reaction–negative samples obtained after treatment of chronic hepatitis C Financial relationships of the authors with Hoffmann-La Roche, Inc., are as follows: T. R. Morgan is consultant, on the speaker's bureau and receives research support; M. L. Shiffman is a consultant, on the speaker's bureau, and receives research support; G. T. Everson is a consultant, on the speaker's bureau, and receives research support; K. L. Lindsay is a consultant and receives research support; W. M. Lee receives research support; and A.S.F. Lok is a consultant and receives research support. Other financial relationships related to this project are: D. R. Gretch, A.S.F. Lok, and W. M. Lee receive research support from Bayer Corporation. Authors with no financial relationships related to this project are: C. Morishima, J. E. Everhart, E. C. Wright, M. Chung, J. L. Dienstag, M. G. Ghany, and T. M. Curto. ." Hepatology 48(5): 1412-1419. <http://hdl.handle.net/2027.42/61324> | en_US |
dc.identifier.issn | 0270-9139 | en_US |
dc.identifier.issn | 1527-3350 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/61324 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=18816437&dopt=citation | en_US |
dc.description.abstract | The Siemens VERSANT® transcription-mediated amplification (TMA) assay is extremely sensitive for the detection of hepatitis C virus (HCV) RNA in serum. Eleven of 180 subjects in the Hepatitis C Antiviral Long-term Treatment against Cirrhosis (HALT-C) Trial who achieved polymerase chain reaction (PCR)-defined sustained virological response (SVR) at week 72 also had TMA-positive results from the same blood draw; six were positive on repeat testing. We report the follow-up on these 11 patients, and the reproducibility of TMA test results from PCR-negative samples in relationship to antiviral treatment outcome. Peginterferon and ribavirin treatment was initiated in 1145 prior interferon nonresponders with advanced hepatic fibrosis. Treatment was continued for 48 weeks if patients had undetectable HCV RNA by PCR at treatment week 20. Frozen serum samples from weeks 12, 20, 24, 48, and 72 were subsequently tested by TMA. Nine of the 11 patients returned for testing (median, 30 months after the week 72 visit), and all had undetectable HCV RNA by TMA and PCR. Among 759 PCR-negative samples obtained during treatment that were tested twice by TMA, 17% overall exhibited consistently positive results, and 21% exhibited inconsistently positive results. SVR was more likely if TMA was consistently negative than if consistently or inconsistently positive. With continued treatment, patients with inconsistently positive TMA results were more likely to become TMA-negative than TMA-positive ( P < 0.0001). Conclusion: In PCR-negative samples, positive TMA results may indicate the presence of low levels of HCV RNA. However, because patients with positive TMA results may achieve SVR, management decisions during therapy should not be based on a single positive TMA test result. (H EPATOLOGY 2008.) | en_US |
dc.format.extent | 136156 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Hepatology | en_US |
dc.title | Interpretation of positive transcription-mediated amplification test results from polymerase chain reaction–negative samples obtained after treatment of chronic hepatitis C | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Internal Medicine and Specialties | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Division of Gastroenterology, University of Michigan Medical Center, Ann Arbor, MI | en_US |
dc.contributor.affiliationother | Virology Division, Department of Laboratory Medicine, University of Washington, Seattle, WA ; fax: 206-744-9858. ; Department of Laboratory Medicine, University of Washington, Box 359690, Seattle, WA 98104 | en_US |
dc.contributor.affiliationother | Division of Gastroenterology, University of California–Irvine, Irvine, CA, and Gastroenterology Service, VA Long Beach Healthcare System, Long Beach, CA | en_US |
dc.contributor.affiliationother | Division of Digestive Diseases and Nutrition, National Institutes of Health, Department of Health and Human Services, Bethesda, MD | en_US |
dc.contributor.affiliationother | Office of the Director, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Department of Health and Human Services, Bethesda, MD | en_US |
dc.contributor.affiliationother | Virology Division, Department of Laboratory Medicine, University of Washington, Seattle, WA | en_US |
dc.contributor.affiliationother | Virology Division, Department of Laboratory Medicine, University of Washington, Seattle, WA | en_US |
dc.contributor.affiliationother | Hepatology Section, Virginia Commonwealth University Medical Center, Richmond, VA | en_US |
dc.contributor.affiliationother | Section of Hepatology, Division of Gastroenterology and Hepatology, University of Colorado School of Medicine, Denver, CO | en_US |
dc.contributor.affiliationother | Division of Gastrointestinal and Liver Diseases, Keck School of Medicine, University of Southern California, Los Angeles, CA | en_US |
dc.contributor.affiliationother | Division of Digestive and Liver Diseases, University of Texas Southwestern Medical Center, Dallas, TX | en_US |
dc.contributor.affiliationother | Gastrointestinal Unit (Medical Services), Massachusetts General Hospital and the Department of Medicine, Harvard Medical School, Boston, MA | en_US |
dc.contributor.affiliationother | Liver Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Department of Health and Human Services, Bethesda, MD | en_US |
dc.contributor.affiliationother | New England Research Institutes, Watertown, MA | en_US |
dc.identifier.pmid | 18816437 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/61324/1/22487_ftp.pdf | |
dc.identifier.doi | http://dx.doi.org/10.1002/hep.22487 | en_US |
dc.identifier.source | Hepatology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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