Nuclear receptor profile in calvarial bone cells undergoing osteogenic versus adipogenic differentiation
dc.contributor.author | Pirih, Flavia Q. | en_US |
dc.contributor.author | Abayahoudian, Rosette | en_US |
dc.contributor.author | Elashoff, David | en_US |
dc.contributor.author | Parhami, Farhad | en_US |
dc.contributor.author | Nervina, Jeanne M. | en_US |
dc.contributor.author | Tetradis, Sotirios | en_US |
dc.date.accessioned | 2008-12-01T21:01:25Z | |
dc.date.available | 2010-01-05T16:59:14Z | en_US |
dc.date.issued | 2008-12-01 | en_US |
dc.identifier.citation | Pirih, Flavia Q.; Abayahoudian, Rosette; Elashoff, David; Parhami, Farhad; Nervina, Jeanne M.; Tetradis, Sotirios (2008). "Nuclear receptor profile in calvarial bone cells undergoing osteogenic versus adipogenic differentiation." Journal of Cellular Biochemistry 105(5): 1316-1326. <http://hdl.handle.net/2027.42/61336> | en_US |
dc.identifier.issn | 0730-2312 | en_US |
dc.identifier.issn | 1097-4644 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/61336 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=18810760&dopt=citation | en_US |
dc.description.abstract | Nuclear receptors (NRs) are key regulators of cell function and differentiation. We examined NR expression during osteogenic versus adipogenic differentiation of primary mouse calvarial osteoblasts (MOBs). MOBs were cultured for 21 days in osteogenic or adipogenic differentiation media. von Kossa and Oil Red O staining, and qRT-PCR of marker genes and 49 NRs were performed. PCR amplicons were subcloned to establish correct sequences and absolute standard curves. Forty-three NRs were detected at days 0–21. Uncentered average linkage hierarchical clustering identified four expression clusters: NRs (1) upregulated during osteogenic, but not adipogenic, differentiation, (2) upregulated in both conditions, with greater upregulation during adipogenic differentiation, (3) upregulated equally in both conditions, (4) downregulated during adipogenic, but not osteogenic, differentiation. One-way ANOVA with contrast revealed 20 NRs upregulated during osteogenic differentiation and 12 NRs upregulated during adipogenic differentiation. Two-way ANOVA demonstrated that 18 NRs were higher in osteogenic media, while 9 NRs were higher in adipogenic media. The time effect revealed 16 upregulated NRs. The interaction of condition with time revealed 6 NRs with higher expression rate during adipogenic differentiation and 3 NRs with higher expression rate during osteogenic differentiation. Relative NR abundance at days 0 and 21 were ranked. Basal ranking changed at least 5 positions for 13 NRs in osteogenic media and 9 NRs in adipogenic media. Osteogenic and adipogenic differentiation significantly altered NR expression in MOBs. These differences offer a fingerprint of cellular commitment and may provide clues to the underlying mechanisms of osteogenic versus adipogenic differentiation. J. Cell. Biochem. 105: 1316–1326, 2008. © 2008 Wiley-Liss, Inc. | en_US |
dc.format.extent | 349553 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Cell & Developmental Biology | en_US |
dc.title | Nuclear receptor profile in calvarial bone cells undergoing osteogenic versus adipogenic differentiation | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Genetics | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationother | Division of Diagnostic and Surgical Sciences, UCLA, Los Angeles, California 90095 | en_US |
dc.contributor.affiliationother | Department of Biostatistics/Nursing, UCLA, Los Angeles, California 90095 | en_US |
dc.contributor.affiliationother | Department of Cardiology, UCLA, Los Angeles, California 90095 | en_US |
dc.contributor.affiliationother | Section of Orthodontics, UCLA, Los Angeles, California 90095 | en_US |
dc.contributor.affiliationother | Division of Diagnostic and Surgical Sciences, UCLA, Los Angeles, California 90095 ; Molecular Biology Institute, UCLA, Los Angeles, California 90095 ; Division of Diagnostic and Surgical Sciences, CHS 53-068, UCLA, School of Dentistry, Los Angeles, CA 90095-1668. | en_US |
dc.identifier.pmid | 18810760 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/61336/1/21931_ftp.pdf | |
dc.identifier.doi | http://dx.doi.org/10.1002/jcb.21931 | en_US |
dc.identifier.source | Journal of Cellular Biochemistry | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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