Now showing items 1-10 of 21
Rapid protein release from Escherichia coli by chemical permeabilization under fermentation conditions
(Wiley Subscription Services, Inc., A Wiley Company, 1992-03-25)
Overall protein release greater than 75% in less than 1 h can be attained by exposing exponentially growing Escherichia coli cells to 0.4 M guanidine plus 0.5% Triton X-100 at 37°C in medium. Cell growth stops immediately ...
Quantitative evaluation of the pH profile in organic acid fermentations
(Wiley Subscription Services, Inc., A Wiley Company, 1991-01-20)
No Abstrct.
Selective extraction using preferential transport through adsorptive membranes
(Wiley Subscription Services, Inc., A Wiley Company, 1996-12-05)
Selective extraction of a protein from a mixture can be accomplished using an adsorptive membrane and low displacement recuperative parametric pumping. Low displacement recuperative parametric pumping can lead to the ...
Molecular analysis of the myosin gene family in Arabidopsis thaliana
(Kluwer Academic Publishers; Springer Science+Business Media, 1994-11)
Myosin is believed to act as the molecular motor for many actin-based motility processes in eukaryotes. It is becoming apparent that a single species may possess multiple myosin isoforms, and at least seven distinct classes ...
Protein extraction using the sodium bis(2-ethylhexyl) phosphate (NaDEHP) reverse micellar system
(Wiley Subscription Services, Inc., A Wiley Company, 1996-04-20)
The reverse micellar system of sodium bis(2-ethylhexyl) phosphate (NaDEHP)/isooctane/brine was used for liquid-liquid extraction of proteins. We investigated the solubilization of cytochrome-c and Α-chymotrypsin into the ...
Degradation kinetics of pentachlorophenol by Phanerochaete chrysosporium
(Wiley Subscription Services, Inc., A Wiley Company, 1990-05)
The extracellular enzymes and cell mass from the pregrown Phanerochaete chrysosporium cultures were used for the degradation of PCP. The use of both extracellular enzymes and cell mass resulted in extensive mineralization ...
1-Monoglyceride production from lipase-catalyzed esterification of glycerol and fatty acid in reverse micelles
(Wiley Subscription Services, Inc., A Wiley Company, 1991-08-20)
Glycerol-fatty acid esterification has been conducted with lipase from R. delemar in water/AOT/isooctane reverse micellar media, with the major product being 1-monoglyceride, a useful food-emulsifier. 1,3-diglyceride was ...
HCCH-TOCSY spectroscopy of 13 C-labeled proteins in H 2 O using heteronuclear cross-polarization and pulsed-field gradients
(Kluwer Academic Publishers; ESCOM Science Publishers B.V. ; Springer Science+Business Media, 1995-02)
A pulsed-field gradient-enhanced, heteronuclear cross-polarization-driven, 3D HCCH-TOCSY experiment is described, which in a single scan can achieve nearly ideal solvent suppression for protein samples in H 2 O solution. ...
Comparative assessment of the chemical and adsorptive characteristics of leachates from a municipal and an industrial landfill
(Kluwer Academic Publishers; Springer Science+Business Media, 1990-10)
Leachates from two landfills, one municipal and one industrial, were compared with respect to their chemical and adsorptive characteristics. Concentrations of most inorganic constituents were as much as an order of magnitude ...
Countercurrent gradient chromatography: A continuous focusing technique
(Wiley Subscription Services, Inc., A Wiley Company, 1995-12-05)
The continuous separation of proteins was performed in a countercurrent gradient chromatography (CGC) system. A magnetically stabilized fluidized bed (MSFB) was used to establish true countercurrent contact of a solid resin ...