Now showing items 1-10 of 47
Rapid protein release from Escherichia coli by chemical permeabilization under fermentation conditions
(Wiley Subscription Services, Inc., A Wiley Company, 1992-03-25)
Overall protein release greater than 75% in less than 1 h can be attained by exposing exponentially growing Escherichia coli cells to 0.4 M guanidine plus 0.5% Triton X-100 at 37°C in medium. Cell growth stops immediately ...
The effects of exopolymers on cell morphology and culturability of Leuconostoc mesenteroides during starvation
(Springer-Verlag; Springer-Verlag Berlin Heidelberg, 1999-11)
Biofilm formation by bacterial cells can be used to modify the subsurface permeability for the purpose of microbial enhanced oil recovery, bio-barrier formation, and in situ bioremediation. Once injected into the subsurface, ...
Quantitative evaluation of the pH profile in organic acid fermentations
(Wiley Subscription Services, Inc., A Wiley Company, 1991-01-20)
No Abstrct.
Recovery of a foreign protein from the periplasm of Escherichia coli by chemical permeabilization
(Elsevier, 1990-08)
We have applied the technique of protein release by chemical permeabilization to recover a foreign protein in active form from the periplasm of a recombinant strain of Escherichia coli. The two agents used in our chemical ...
Selective extraction using preferential transport through adsorptive membranes
(Wiley Subscription Services, Inc., A Wiley Company, 1996-12-05)
Selective extraction of a protein from a mixture can be accomplished using an adsorptive membrane and low displacement recuperative parametric pumping. Low displacement recuperative parametric pumping can lead to the ...
Protein separation using membrane-encapsulated soluble ligand conjugates
(Elsevier, 1990-05)
A new approach for isolating and recovering biological macromolecules using membrane-encapsulated soluble ligand conjugates was investigated. Membrane-encapsulated solid adsorbents have been successfully developed and ...
Cell affinity separations using magnetically stabilized fluidized beds: Erythrocyte subpopulation fractionation utilizing a lectin-magnetite support
(Wiley Subscription Services, Inc., A Wiley Company, 2003-03-20)
A magnetically stabilized fluidized bed is used to separate erythrocyte subpopulations. Binding specificity was obtained by immobilizing the lectin Helix pomatia Agglutinin (HpA) or Griffonia simplicifolia I (GSI) onto ...
Pore-scale investigation of biomass plug development and propagation in porous media
(John Wiley & Sons, Inc., 2002-03-05)
Biomass plugging of porous media finds application in enhanced oil recovery and bioremediation. An understanding of biomass plugging of porous media was sought by using a porous glass micromodel through which biomass and ...
Growth of Leuconostoc mesenteroides NRRL-B523 in an alkaline medium: Suboptimal pH growth inhibition of a lactic acid bacterium
(Wiley Subscription Services, Inc., A Wiley Company, 2005-01-05)
Bacterial profile modification (BPM), a form of tertiary oil recovery, diverts water from the water-flooded high-permeability zone into the oil-bearing low-permeability zone. During field use, exopolymer-producing bacteria ...
Biomass evolution in porous media and its effects on permeability under starvation conditions
(John Wiley & Sons, Inc., 2000-07-05)
The purpose of this study was to understand bacteria profile modification and its applications in subsurface biological operations such as biobarrier formation, in situ bioremediation, and microbial-enhanced oil recovery. ...