Now showing items 1-10 of 11
The effect of feedback regulation and in situ product removal on the conversion of sugar to cycloheximide by Streptomyces griseus
(Springer-Verlag, 1989-03)
An addition of cycloheximide to cycloheximide-producing Streptomyces griseus cultures resulted in reductions in the production rate and in the conversion of sugar into cycloheximide. In situ cycloheximide adsorption was ...
Computer aided analysis for biosensing and screening
(Wiley Subscription Services, Inc., A Wiley Company, 1988-02-20)
Biosensors with animal and microbial cells immobilized close to the tip of a membrane electrode have been developed for chemical and drug testing. Our experimental results show that biosensors can be used for drug screening ...
Fermentation monitoring by polymeric-resin-based microbiol assay
(Wiley Subscription Services, Inc., A Wiley Company, 1983-03)
A polymeric-resin-based microbiological assay is shown to yield results consistent with classical inhibition zone theory. This novel assay is applicable to cycloheximide, an antifungal antibiotic, at concentrations below ...
Cell immobilization in k -carrageenan with tricalcium phosphate
(Wiley Subscription Services, Inc., A Wiley Company, 1982-08)
The objectives of this research were to investigate the growth of immobilized yeast cells in k -carrageenan gel and study the effect of trapping hydroxyapatite (tricalcium phosphate) crystals into the matrix. Using k ...
Computer modeling of antibiotic fermentation with on-line product removal
(Wiley Subscription Services, Inc., A Wiley Company, 1988-07-20)
The fermentation of Streptomyces griseus for the production of cycloheximide is similar to other antibiotic fermentations in that product synthesis is subject to feedback regulation and the desired product is degraded ...
Protein engineering of penicillinase as affinity ligands for bioprocessing
(Elsevier, 1989)
The active site and the substrate binding site of penicillinase ([beta]-lactamase) from Bacillus licheniformis were altered in this study so that the enzyme retains the specific binding capability to the [beta]-lactam ...
Modeling nutrient behavior in Wetlands
(Elsevier, 1988-01)
A simple mathematical model os developed which permites dynamic simulation of wetland hydrology and of nutrient-driven interactions between wastewater and the wetland ecosystem. Spatial variations due to surface water flow ...
Use of modified clays for the removal and disposal of chlorinated dioxins and other priority pollutants from industrial wastewaters
(Elsevier, 1989)
The dynamics of adsorption and elution of octachlorodibenzodioxin (OCDD) on modified clay adsorbents has been studied. In batch type experiments, a rapid approach to sorption equilibrium (within hours) as well as a Freudlich ...
A new isolation and purification method for staphylococcal protein A using membrane encapsulated rabbit IgG-agarose
(Wiley Subscription Services, Inc., A Wiley Company, 1989-10-20)
A new isolation and purification method for bioproducts using membrane-encapsulated affinity adsorbents was investigated. The new method involves encapsulation of affinity adsorbents, batch adsorption of the bioproduct ...
Protein release from Escherichia coli cells permeabilized with guanidine–HCl and Triton X100
(Wiley Subscription Services, Inc., A Wiley Company, 1989-02-20)
An important factor complicating the recovery of recombinant proteins from Escherichia coli is their intracellular location. An alternative to the commonly used method of releasing these proteins by mechanical disruption ...