Now showing items 1-10 of 15
Rapid protein release from Escherichia coli by chemical permeabilization under fermentation conditions
(Wiley Subscription Services, Inc., A Wiley Company, 1992-03-25)
Overall protein release greater than 75% in less than 1 h can be attained by exposing exponentially growing Escherichia coli cells to 0.4 M guanidine plus 0.5% Triton X-100 at 37°C in medium. Cell growth stops immediately ...
Quantitative evaluation of the pH profile in organic acid fermentations
(Wiley Subscription Services, Inc., A Wiley Company, 1991-01-20)
No Abstrct.
Selective extraction using preferential transport through adsorptive membranes
(Wiley Subscription Services, Inc., A Wiley Company, 1996-12-05)
Selective extraction of a protein from a mixture can be accomplished using an adsorptive membrane and low displacement recuperative parametric pumping. Low displacement recuperative parametric pumping can lead to the ...
Protein extraction using the sodium bis(2-ethylhexyl) phosphate (NaDEHP) reverse micellar system
(Wiley Subscription Services, Inc., A Wiley Company, 1996-04-20)
The reverse micellar system of sodium bis(2-ethylhexyl) phosphate (NaDEHP)/isooctane/brine was used for liquid-liquid extraction of proteins. We investigated the solubilization of cytochrome-c and Α-chymotrypsin into the ...
Degradation kinetics of pentachlorophenol by Phanerochaete chrysosporium
(Wiley Subscription Services, Inc., A Wiley Company, 1990-05)
The extracellular enzymes and cell mass from the pregrown Phanerochaete chrysosporium cultures were used for the degradation of PCP. The use of both extracellular enzymes and cell mass resulted in extensive mineralization ...
1-Monoglyceride production from lipase-catalyzed esterification of glycerol and fatty acid in reverse micelles
(Wiley Subscription Services, Inc., A Wiley Company, 1991-08-20)
Glycerol-fatty acid esterification has been conducted with lipase from R. delemar in water/AOT/isooctane reverse micellar media, with the major product being 1-monoglyceride, a useful food-emulsifier. 1,3-diglyceride was ...
Countercurrent gradient chromatography: A continuous focusing technique
(Wiley Subscription Services, Inc., A Wiley Company, 1995-12-05)
The continuous separation of proteins was performed in a countercurrent gradient chromatography (CGC) system. A magnetically stabilized fluidized bed (MSFB) was used to establish true countercurrent contact of a solid resin ...
Formation of polyol–fatty acid esters by lipases in reverse micellar media
(Wiley Subscription Services, Inc., A Wiley Company, 1992-06-05)
The synthesis of polyol–fatty acid esters has strong implications in such industries as foods, cosmetics, and polymers. We have investigated these esterification reactions employing the polyols ethylene glycol, 2-monoglyceride, ...
Application of membrane-based preferential transport to whole broth processing
(Wiley Subscription Services, Inc., A Wiley Company, 1997-08-20)
Preferential transport in adsorptive membranes can be used to selectively remove biochemicals directly from fermentation broths. During preferential transport, an adsorbing solute is selectively transported across the ...
Use of coimmobilized biological systems to degrade toxic organic compounds
(Wiley Subscription Services, Inc., A Wiley Company, 1991-07)
The concept of coimmobilizing cell mass (and/or enzyme) and adsorbent in a hydrogel matrix for biodegradation of toxic organic chemicals was introduced. Under defined experimental conditions, the coimmobilized system using ...