Transverse imaging and simulation of dsDNA electrophoresis in microfabricated glass channels
dc.contributor.author | Zeitoun, Ramsey I. | en_US |
dc.contributor.author | Chen, Zheng | en_US |
dc.contributor.author | Burns, Mark A. | en_US |
dc.date.accessioned | 2009-01-07T15:29:34Z | |
dc.date.available | 2010-02-02T15:48:07Z | en_US |
dc.date.issued | 2008-12 | en_US |
dc.identifier.citation | Zeitoun, Ramsey I.; Chen, Zheng; Burns, Mark A. (2008). "Transverse imaging and simulation of dsDNA electrophoresis in microfabricated glass channels." Electrophoresis 29(23): 4768-4774. <http://hdl.handle.net/2027.42/61440> | en_US |
dc.identifier.issn | 0173-0835 | en_US |
dc.identifier.issn | 1522-2683 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/61440 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=19053075&dopt=citation | en_US |
dc.description.abstract | We have observed the non-uniform distribution of DNA molecules during PAGE in a microfabricated system. Confocal laser scanning microscopy was used to visualize fluorescently labeled DNA during electrophoretic migration. The distribution of double-stranded DNA larger than 100 bp is observed to transition from a center-biased motion on the transverse plane 1 cm downstream from injection to an edge-biased motion 2 cm downstream. Although this distribution increased with increasing dsDNA size in a cross-linked gel, no similar distribution was found with the same dsDNA molecules in a linear polyacrylamide solution (6%). Simulations of DNA distribution in gels suggest that DNA distribution non-uniformities may be caused by biased electrophoretic migration resulting from motion in an inhomogeneous gel system. | en_US |
dc.format.extent | 279514 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | WILEY-VCH Verlag | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Biochemistry and Biotechnology | en_US |
dc.title | Transverse imaging and simulation of dsDNA electrophoresis in microfabricated glass channels | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Chemical Engineering, The University of Michigan, Ann Arbor, MI, USA ; These authors contributed equally to this work. | en_US |
dc.contributor.affiliationum | Department of Chemical Engineering, The University of Michigan, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Department of Chemical Engineering, The University of Michigan, Ann Arbor, MI, USA ; Department of Biomedical Engineering, The University of Michigan, Ann Arbor, MI, USA ; Department of Chemical Engineering, The University of Michigan, Ann Arbor, MI 48109, USA Fax: +1-734-763-0459 | en_US |
dc.identifier.pmid | 19053075 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/61440/1/4768_ftp.pdf | |
dc.identifier.doi | http://dx.doi.org/10.1002/elps.200800204 | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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