Cre-mediated recombination in pituitary somatotropes
dc.contributor.author | Nasonkin, Igor O. | en_US |
dc.contributor.author | Potok, Mary Anne | en_US |
dc.contributor.author | Camper, Sally A. | en_US |
dc.date.accessioned | 2009-02-03T16:16:27Z | |
dc.date.available | 2010-03-01T21:10:28Z | en_US |
dc.date.issued | 2009-01 | en_US |
dc.identifier.citation | Nasonkin, Igor O.; Potok, Mary Anne; Camper, Sally A. (2009). "Cre-mediated recombination in pituitary somatotropes." genesis 47(1): 55-60. <http://hdl.handle.net/2027.42/61524> | en_US |
dc.identifier.issn | 1526-954X | en_US |
dc.identifier.issn | 1526-968X | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/61524 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=19039787&dopt=citation | en_US |
dc.description.abstract | We report a transgenic line with highly penetrant cre recombinase activity in the somatotrope cells of the anterior pituitary gland. Expression of the cre transgene is under the control of the locus control region of the human growth hormone gene cluster and the rat growth hormone promoter. Cre recombinase activity was assessed with two different lacZ reporter genes that require excision of a floxed stop sequence for expression: a chick Β-actin promoter with the CMV enhancer transgene and a ROSA26 knock-in. Cre activity is detectable in the developing pituitary after initiation of Gh transcription and persists through adulthood with high penetrance in Gh expressing cells and lower penetrance in lactotropes, a cell type that shares a common origin with somatotropes. This Gh-cre transgenic line is suitable for efficient, cell-specific deletion of floxed regions of genomic DNA in differentiated somatotropes and a subset of lactotrope cells of the anterior pituitary gland. genesis 47:55–60, 2009. © 2008 Wiley-Liss, Inc. | en_US |
dc.format.extent | 417431 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Genetics | en_US |
dc.title | Cre-mediated recombination in pituitary somatotropes | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Genetics | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Human Genetics, University of Michigan, Ann Arbor, Michigan ; Igor O. Nasonkin and Mary Anne Potok have contributed equally to this work. | en_US |
dc.contributor.affiliationum | Department of Human Genetics, University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Human Genetics, University of Michigan, Ann Arbor, Michigan ; Department of Human Genetics, University of Michigan Medical School, 4909 Buhl Building, 1241 Catherine Street, Ann Arbor, MI 48109-5618 | en_US |
dc.identifier.pmid | 19039787 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/61524/1/20462_ftp.pdf | |
dc.identifier.doi | http://dx.doi.org/10.1002/dvg.20462 | en_US |
dc.identifier.source | genesis | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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