Calcium and calcium sensing receptor modulates the expression of thymidylate synthase, NAD(P)H:quinone oxidoreductase 1 and survivin in human colon carcinoma cells: Promotion of cytotoxic response to mitomycin C and fluorouracil
dc.contributor.author | Liu, Guangming | en_US |
dc.contributor.author | Hu, Xin | en_US |
dc.contributor.author | Varani, James | en_US |
dc.contributor.author | Chakrabarty, Subhas | en_US |
dc.date.accessioned | 2009-03-03T20:08:05Z | |
dc.date.available | 2010-04-14T17:40:05Z | en_US |
dc.date.issued | 2009-03 | en_US |
dc.identifier.citation | Liu, Guangming; Hu, Xin; Varani, James; Chakrabarty, Subhas (2009). "Calcium and calcium sensing receptor modulates the expression of thymidylate synthase, NAD(P)H:quinone oxidoreductase 1 and survivin in human colon carcinoma cells: Promotion of cytotoxic response to mitomycin C and fluorouracil." Molecular Carcinogenesis 48(3): 202-211. <http://hdl.handle.net/2027.42/61862> | en_US |
dc.identifier.issn | 0899-1987 | en_US |
dc.identifier.issn | 1098-2744 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/61862 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=18618519&dopt=citation | en_US |
dc.description.abstract | Ca 2+ and the cell-surface calcium sensing receptor (CaSR) constitute a novel and robust ligand/receptor system in regulating the proliferation and differentiation of colonic epithelial cells. Here we show that activation of CaSR by extracellular Ca 2+ (or CaSR agonists) enhanced the sensitivity of human colon carcinoma cells to mitomycin C (MMC) and fluorouracil (5-FU). Activation of CaSR up-regulated the expression of MMC activating enzyme, NAD(P)H:quinone oxidoreductase 1 (NQO-1) and down-regulated the expression of 5-FU target, thymidylate synthase (TS) and the anti-apoptotic protein survivin. Cells that were resistant to drugs expressed little or no CaSR but abundant amount of survivin. Disruption of CaSR expression by shRNA targeting the CaSR abrogated these modulating effects of CaSR activation on the expression of NQO1, TS, survivin and cytotoxic response to drugs. It is concluded that activation of CaSR can enhance colon cancer cell sensitivity to MMC and 5-FU and can modulate the expression of molecules involved in the cellular responses to these cytotoxic drugs. © 2008 Wiley-Liss, Inc. | en_US |
dc.format.extent | 280561 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Cancer Research, Oncology and Pathology | en_US |
dc.title | Calcium and calcium sensing receptor modulates the expression of thymidylate synthase, NAD(P)H:quinone oxidoreductase 1 and survivin in human colon carcinoma cells: Promotion of cytotoxic response to mitomycin C and fluorouracil | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Internal Medicine and Specialties | en_US |
dc.subject.hlbsecondlevel | Oncology and Hematology | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationother | Southern Illinois University School of Medicine, SimmonsCooper Cancer Institute, Springfield, Illinois | en_US |
dc.contributor.affiliationother | Southern Illinois University School of Medicine, SimmonsCooper Cancer Institute, Springfield, Illinois | en_US |
dc.contributor.affiliationother | Southern Illinois University School of Medicine, SimmonsCooper Cancer Institute, Springfield, Illinois ; Department of Microbiology, Immunology and Cell Biology, Southern Illinois University School of Medicine, SimmonsCooper Cancer Institute, Springfield, IL 62794-9677. | en_US |
dc.identifier.pmid | 18618519 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/61862/1/20470_ftp.pdf | |
dc.identifier.doi | 10.1002/mc.20470 | en_US |
dc.identifier.source | Molecular Carcinogenesis | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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