Reverse remodeling is associated with changes in extracellular matrix proteases and tissue inhibitors after mesenchymal stem cell (MSC) treatment of pressure overload hypertrophy
dc.contributor.author | Molina, Ezequiel J. | en_US |
dc.contributor.author | Palma, Jon | en_US |
dc.contributor.author | Gupta, Dipin | en_US |
dc.contributor.author | Torres, Denise | en_US |
dc.contributor.author | Gaughan, John P. | en_US |
dc.contributor.author | Houser, Steven | en_US |
dc.contributor.author | Macha, Mahender | en_US |
dc.date.accessioned | 2009-03-03T20:11:22Z | |
dc.date.available | 2010-04-14T17:40:06Z | en_US |
dc.date.issued | 2009-02 | en_US |
dc.identifier.citation | Molina, Ezequiel J.; Palma, Jon; Gupta, Dipin; Torres, Denise; Gaughan, John P.; Houser, Steven; Macha, Mahender (2009). "Reverse remodeling is associated with changes in extracellular matrix proteases and tissue inhibitors after mesenchymal stem cell (MSC) treatment of pressure overload hypertrophy." Journal of Tissue Engineering and Regenerative Medicine 3(2): 85-91. <http://hdl.handle.net/2027.42/61900> | en_US |
dc.identifier.issn | 1932-6254 | en_US |
dc.identifier.issn | 1932-7005 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/61900 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=19065545&dopt=citation | en_US |
dc.description.abstract | Changes in ventricular extracellular matrix (ECM) composition of pressure overload hypertrophy determine clinical outcomes. The effects of mesenchymal stem cell (MSC) transplantation upon determinants of ECM composition in pressure overload hypertrophy have not been studied. Sprague–Dawley rats underwent aortic banding and were followed by echocardiography. After an absolute decrease in fractional shortening of 25% from baseline, 1 × 10 6 MSC ( n = 28) or PBS ( n = 20) was randomly injected intracoronarily. LV protein analysis, including matrix metalloproteinases (MMP-2, MMP-3, MMP-6, MMP-9) and tissue inhibitors of metalloproteinases (TIMP-1, TIMP-2, TIMP-3), was performed after sacrifice on postoperative day 7, 14, 21 or 28. Left ventricular levels of MMP-3, MMP-6, MMP-9, TIMP-1 and TIMP-3 were demonstrated to be decreased in the MSC group compared with controls after 28 days. Expression of MMP-2 and TIMP-2 remained relatively stable in both groups. Successful MSCs delivery was confirmed by histological analysis and visualization of labelled MSCs. In this model of pressure overload hypertrophy, intracoronary delivery of MSCs during heart failure was associated with specific changes in determinants of ECM composition. LV reverse remodeling was associated with decreased ventricular levels of MMP-3, MMP-6, MMP-9, TIMP-1 and TIMP-3, which were upregulated in the control group as heart failure progressed. These effects were most significant at 28 days following injection. Copyright © 2008 John Wiley & Sons, Ltd. | en_US |
dc.format.extent | 199137 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | John Wiley & Sons, Ltd. | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Medicine | en_US |
dc.title | Reverse remodeling is associated with changes in extracellular matrix proteases and tissue inhibitors after mesenchymal stem cell (MSC) treatment of pressure overload hypertrophy | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Medicine (General) | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Division of Cardiac Surgery, University of Michigan Medical School, Ann Arbor, MI, USA ; Suite 301, 1100 E. Michigan Avenue, Jackson, MI 49201, USA. | en_US |
dc.contributor.affiliationother | Division of Cardiac and Thoracic Surgery, Temple University School of Medicine, Philadelphia, PA, USA | en_US |
dc.contributor.affiliationother | Division of Cardiac and Thoracic Surgery, Temple University School of Medicine, Philadelphia, PA, USA | en_US |
dc.contributor.affiliationother | Division of Cardiac and Thoracic Surgery, Temple University School of Medicine, Philadelphia, PA, USA | en_US |
dc.contributor.affiliationother | Division of Cardiac and Thoracic Surgery, Temple University School of Medicine, Philadelphia, PA, USA | en_US |
dc.contributor.affiliationother | Department of Physiology, Temple University School of Medicine, Philadelphia, PA, USA | en_US |
dc.contributor.affiliationother | Department of Physiology, Temple University School of Medicine, Philadelphia, PA, USA | en_US |
dc.identifier.pmid | 19065545 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/61900/1/137_ftp.pdf | |
dc.identifier.doi | 10.1002/term.137 | en_US |
dc.identifier.source | Journal of Tissue Engineering and Regenerative Medicine | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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