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Accurate multiplex gene synthesis from programmable DNA microchips

dc.contributor.authorTian, J. D.en_US
dc.contributor.authorGong, H.en_US
dc.contributor.authorSheng, N. J.en_US
dc.contributor.authorZhou, X. C.en_US
dc.contributor.authorGulari, Erdoganen_US
dc.contributor.authorGao, Xiaolianen_US
dc.contributor.authorChurch, G.en_US
dc.date.accessioned2009-06-01T17:31:00Z
dc.date.available2009-06-01T17:31:00Z
dc.date.issued2004-12-23en_US
dc.identifier.citationTian, JD; Gong, H; Sheng, NJ; Zhou, XC; Gulari, E; Gao, XL; Church, G. (2004) "Accurate multiplex gene synthesis from programmable DNA microchips." Nature 432(7020): 1050-1054. <http://hdl.handle.net/2027.42/62677>en_US
dc.identifier.issn0028-0836en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/62677
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=15616567&dopt=citationen_US
dc.description.abstractTesting the many hypotheses from genomics and systems biology experiments demands accurate and cost-effective gene and genome synthesis. Here we describe a microchip-based technology for multiplex gene synthesis. Pools of thousands of 'construction' oligonucleotides and tagged complementary 'selection' oligonucleotides are synthesized on photo-programmable microfluidic chips(1), released, amplified and selected by hybridization to reduce synthesis errors ninefold. A one-step polymerase assembly multiplexing reaction assembles these into multiple genes. This technology enabled us to synthesize all 21 genes that encode the proteins of the Escherichia coli 30S ribosomal subunit, and to optimize their translation efficiency in vitro through alteration of codon bias. This is a significant step towards the synthesis of ribosomes in vitro and should have utility for synthetic biology in general.en_US
dc.format.extent244929 bytes
dc.format.extent2489 bytes
dc.format.mimetypeapplication/octet-stream
dc.format.mimetypetext/plain
dc.publisherNature Publishing Groupen_US
dc.sourceNatureen_US
dc.titleAccurate multiplex gene synthesis from programmable DNA microchipsen_US
dc.typeArticleen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumUniv Michigan, Dept Chem Engn, Ann Arbor, MI 48109 USAen_US
dc.contributor.affiliationotherHarvard Univ, Sch Med, Boston, MA 02115 USAen_US
dc.contributor.affiliationotherUniv Houston, Dept Chem, Houston, TX 77004 USAen_US
dc.contributor.affiliationotherAtact Technol Inc, Houston, TX 77054 USAen_US
dc.identifier.pmid15616567en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/62677/1/nature03151.pdf
dc.identifier.doihttp://dx.doi.org/10.1038/nature03151en_US
dc.identifier.sourceNatureen_US
dc.contributor.authoremailglmlcl@arep.med.harvard.eduen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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