Cloning Of An Nf-kappa-b Subunit Which Stimulates Hiv Transcription In Synergy With P65
dc.contributor.author | Schmid, Roland M. | en_US |
dc.contributor.author | Perkins, Neil D. | en_US |
dc.contributor.author | Duckett, C. S. | en_US |
dc.contributor.author | Andrews, P. C. | en_US |
dc.contributor.author | Nabel, Gary J. | en_US |
dc.date.accessioned | 2009-06-01T17:39:44Z | |
dc.date.available | 2009-06-01T17:39:44Z | |
dc.date.issued | 1991-08-22 | en_US |
dc.identifier.citation | Schmid, RM; Perkins, ND; Duckett, CS; Andrews, PC; Nabel, GJ. (1991) "Cloning Of An Nf-kappa-b Subunit Which Stimulates Hiv Transcription In Synergy With P65." Nature 352(6337): 733-736. <http://hdl.handle.net/2027.42/62829> | en_US |
dc.identifier.issn | 0028-0836 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/62829 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1876189&dopt=citation | en_US |
dc.description.abstract | THE transcription factor NF-kappa-B is a protein complex which comprises a DNA-binding subunit and an associated transactivation protein (of relative molecular masses 50,000 (50K) and 65K, respectively) 1,2. Both the 50K and 65K subunits have similarity with the rel oncogene and the Drosophila maternal effect gene dorsal 3-6. The 50K DNA-binding subunit was previously thought to be a unique protein, derived from the 105K gene product (p105). We now report the isolation of a complementary DNA that encodes an alternative DNA-binding subunit of NF-kappa-B. It is more similar to p105 NF-kappa-B than other family members and defines a new subset of rel-related genes. It is synthesized as a approximately 100K protein (p100) that is expressed in different cell types, contains cell cycle motifs and, like p105, must be processed to generate a 50K form. A 49K product (p49) can be generated independently from an alternatively spliced transcript; it has specific kappa-B DNA-binding activity and can form heterodimers with other rel proteins. In contrast to the approximately 50K protein derived from p105, p49 acts in synergy with p65 to stimulate the human immunodeficiency virus (HIV) enhancer in transiently transfected Jurkat cells. p49/p100 NF-kappa-B could therefore be important in the regulation of HIV and other kappa-B-containing genes. | en_US |
dc.format.extent | 590252 bytes | |
dc.format.extent | 2489 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Macmillan Magazines Ltd. | en_US |
dc.source | Nature | en_US |
dc.title | Cloning Of An Nf-kappa-b Subunit Which Stimulates Hiv Transcription In Synergy With P65 | en_US |
dc.type | Article | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | UNIV MICHIGAN,MED CTR,HOWARD HUGHES MED INST,DEPT INTERNAL MED,ANN ARBOR,MI 48109 | en_US |
dc.contributor.affiliationum | UNIV MICHIGAN,MED CTR,HOWARD HUGHES MED INST,DEPT BIOL CHEM,ANN ARBOR,MI 48109 | en_US |
dc.identifier.pmid | 1876189 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/62829/1/352733a0.pdf | |
dc.identifier.doi | http://dx.doi.org/10.1038/352733a0 | en_US |
dc.identifier.source | Nature | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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