Quantitative inference of cellular parameters from microfluidic cell culture systems
dc.contributor.author | Mehta, Khamir H. | en_US |
dc.contributor.author | Mehta, Geeta | en_US |
dc.contributor.author | Takayama, Shuichi | en_US |
dc.contributor.author | Linderman, Jennifer J. | en_US |
dc.date.accessioned | 2009-07-06T15:38:06Z | |
dc.date.available | 2010-10-05T18:27:29Z | en_US |
dc.date.issued | 2009-08-01 | en_US |
dc.identifier.citation | Mehta, Khamir; Mehta, Geeta; Takayama, Shuichi; Linderman, Jennifer (2009). "Quantitative inference of cellular parameters from microfluidic cell culture systems." Biotechnology and Bioengineering 103(5): 966-974. <http://hdl.handle.net/2027.42/63052> | en_US |
dc.identifier.issn | 0006-3592 | en_US |
dc.identifier.issn | 1097-0290 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/63052 | |
dc.description.abstract | Microfluidic cell culture systems offer a convenient way to measure cell biophysical parameters in conditions close to the physiological environment. We demonstrate the application of a mathematical model describing the spatial distribution of nutrient and growth factor concentrations in inferring cellular oxygen uptake rates from experimental measurements. We use experimental measurements of oxygen concentrations in a poly(dimethylsiloxane) (PDMS) microreactor culturing human hepatocellular liver carcinoma cells (HepG2) to infer quantitative information on cellular oxygen uptake rates. We use a novel microchannel design to avoid the parameter correlation problem associated with simultaneous cellular uptake and diffusion of oxygen through the PDMS surface. We find that the cellular uptake of oxygen is dependent on the cell density and can be modeled using a logistic term in the Michaelis–Menten equation. Our results are significant not only for the development of novel assays to quantitatively infer cell response to stimuli, but also for the development, design, and optimization of novel in vitro systems for drug discovery and tissue engineering. Biotechnol. Bioeng. 2009;103: 966–974. © 2009 Wiley Periodicals, Inc. | en_US |
dc.format.extent | 381586 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Biochemistry and Biotechnology | en_US |
dc.title | Quantitative inference of cellular parameters from microfluidic cell culture systems | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbsecondlevel | Mathematics | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Statistics and Numeric Data | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Social Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Chemical Engineering, University of Michigan, 3328 G.G. Brown, 2300 Hayward, Ann Arbor, Michigan 48109; telephone: 734-763-0679; fax: 734-764-7453 | en_US |
dc.contributor.affiliationum | Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Chemical Engineering, University of Michigan, 3328 G.G. Brown, 2300 Hayward, Ann Arbor, Michigan 48109; telephone: 734-763-0679; fax: 734-764-7453 ; Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan ; Department of Chemical Engineering, University of Michigan, 3328 G.G. Brown, 2300 Hayward, Ann Arbor, Michigan 48109; telephone: 734-763-0679; fax: 734-764-7453. | en_US |
dc.identifier.pmid | 19388086 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/63052/1/22334_ftp.pdf | |
dc.identifier.doi | 10.1002/bit.22334 | en_US |
dc.identifier.source | Biotechnology and Bioengineering | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.