Localization of IFN-γ-Activated Stat1 and IFN Regulatory Factors 1 and 2 in Breast Cancer Cells
dc.contributor.author | Connett, Judith M. | en_US |
dc.contributor.author | Hunt, Steven R. | en_US |
dc.contributor.author | Hickerson, Suzanne M. | en_US |
dc.contributor.author | Wu, Susan J. | en_US |
dc.contributor.author | Doherty, Gerard M. | en_US |
dc.date.accessioned | 2009-07-10T18:59:29Z | |
dc.date.available | 2009-07-10T18:59:29Z | |
dc.date.issued | 2003-11-01 | en_US |
dc.identifier.citation | Connett, Judith M.; Hunt, Steven R.; Hickerson, Suzanne M.; Wu, Susan J.; Doherty, Gerard M. (2003). "Localization of IFN-γ-Activated Stat1 and IFN Regulatory Factors 1 and 2 in Breast Cancer Cells." Journal of Interferon & Cytokine Research 23(11): 621-630 <http://hdl.handle.net/2027.42/63148> | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/63148 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=14651776&dopt=citation | en_US |
dc.description.abstract | The aim of the present work was to evaluate the induction and localization of Stat1, interferon (IFN) regulatory factor-1 (IRF-1), and IRF-2 after IFN-γ exposure of human breast cancer cell lines, SKBR3, MDA468, MCF7, and BT20. Results from growth assays, Western staining, electrophoretic mobility shift assay (EMSA), and immunohistochemical staining were collated to test our hypothesis that immunohistochemical analysis of Stat1, IRF-1, and IRF-2 would provide additional information about the functionality of the IFN-γ signaling pathway in human tumor lines. EMSA results showed that in each of four cell lines, Stat1 expression was increased and demonstrated functional activity after IFN-γ stimulation. Western and EMSA analysis showed upregulation of IRF-1 but not IRF-2 in each cell line. Confocal microscopy of cells stained for Stat1, IRF-1, and IRF-2 confirmed the results and also provided novel information about the intracellular localization of proteins and intercellular variations in responses. The proportion of cells with IRF-1 stimulation and translocation was positively correlated with the IFN-γ growth suppression in vitro. In conclusion, using four independent assays, we have demonstrated that heterogeneity in IFN-γ-mediated upregulation of signal transduction proteins can be detected in vitro and that these differences can explain distinct cellular growth effects. | en_US |
dc.format.extent | 900659 bytes | |
dc.format.extent | 2489 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Mary Ann Liebert, Inc., publishers | en_US |
dc.title | Localization of IFN-γ-Activated Stat1 and IFN Regulatory Factors 1 and 2 in Breast Cancer Cells | en_US |
dc.type | Article | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.identifier.pmid | 14651776 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/63148/1/107999003322558755.pdf | |
dc.identifier.doi | doi:10.1089/107999003322558755 | en_US |
dc.identifier.source | Journal of Interferon & Cytokine Research | en_US |
dc.identifier.source | Journal of Interferon & Cytokine Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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