Role of Integrin Expression in Adenovirus-Mediated Gene Delivery to the Intestinal Epithelium
dc.contributor.author | Croyle, Maria A. | en_US |
dc.contributor.author | Walter, Elke | en_US |
dc.contributor.author | Janich, Sonia L. | en_US |
dc.contributor.author | Roessler, Blake J. | en_US |
dc.contributor.author | Amidon, Gordon L. | en_US |
dc.date.accessioned | 2009-07-10T19:00:10Z | |
dc.date.available | 2009-07-10T19:00:10Z | |
dc.date.issued | 1998-03-01 | en_US |
dc.identifier.citation | Croyle, Maria A.; Walter, Elke; Janich, Sonia; Roessler, Blake J.; Amidon, Gordon L. (1998). "Role of Integrin Expression in Adenovirus-Mediated Gene Delivery to the Intestinal Epithelium." Human Gene Therapy 9(4): 561-573 <http://hdl.handle.net/2027.42/63160> | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/63160 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=9525317&dopt=citation | en_US |
dc.description.abstract | Overview summary Previous studies of adenovirus-mediated gene transfer of the interleukin-1 receptor antagonist protein to rat jejunum have produced limited gene expression. In the present study, we have shown that integrins play a significant role in efficient adenoviral infection of the intestinal epithelium. As enterocytes differentiate, integrin expression decreases. This coincides with significant reduction in adenoviral transduction efficiency. Results from two in vitro models of the intestinal epithelium (Caco-2 and IEC-18 cells) show that αvβ3 integrins have the most influence on adenoviral internalization. Results from screening of rat intestinal segments for expression of the αvβ5 integrin suggest that, based on integrin expression, the ileum is a prime target for efficient adenovirus-mediated gene transfer. We have also found that administration of immunomodulating agents and inflammatory disease states provide a favorable environment for efficient internalization of adenoviral vectors due to up-regulation of integrin receptors. | en_US |
dc.format.extent | 1473359 bytes | |
dc.format.extent | 2489 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Mary Ann Liebert, Inc., publishers | en_US |
dc.title | Role of Integrin Expression in Adenovirus-Mediated Gene Delivery to the Intestinal Epithelium | en_US |
dc.type | Article | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.identifier.pmid | 9525317 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/63160/1/hum.1998.9.4-561.pdf | |
dc.identifier.doi | doi:10.1089/hum.1998.9.4-561 | en_US |
dc.identifier.source | Human Gene Therapy | en_US |
dc.identifier.source | Human Gene Therapy | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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