Induction of Cytotoxic Oxidative Stress by d-Alanine in Brain Tumor Cells Expressing Rhodotorula gracilis d-Amino Acid Oxidase: A Cancer Gene Therapy Strategy
dc.contributor.author | Stegman, Lauren D. | en_US |
dc.contributor.author | Zheng, Hong | en_US |
dc.contributor.author | Neal, Eric R. | en_US |
dc.contributor.author | Ben-Yoseph, Oded | en_US |
dc.contributor.author | Pollegioni, Loredano | en_US |
dc.contributor.author | Pilone, Mirella S. | en_US |
dc.contributor.author | Ross, Brian D. | en_US |
dc.date.accessioned | 2009-07-10T19:03:32Z | |
dc.date.available | 2009-07-10T19:03:32Z | |
dc.date.issued | 1998-01-20 | en_US |
dc.identifier.citation | Stegman, Lauren D.; Zheng, Hong; Neal, Eric R.; Ben-Yoseph, Oded; Pollegioni, Loredano; Pilone, Mirella S.; Ross, Brian D. (1998). "Induction of Cytotoxic Oxidative Stress by d-Alanine in Brain Tumor Cells Expressing Rhodotorula gracilis d-Amino Acid Oxidase: A Cancer Gene Therapy Strategy." Human Gene Therapy 9(2): 185-193 <http://hdl.handle.net/2027.42/63220> | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/63220 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=9472778&dopt=citation | en_US |
dc.description.abstract | Overview summary Gene-directed enzyme prodrug therapy (GDEPT) is an antineoplastic treatment strategy designed to overcome the systemic toxicity of chemotherapy by specifically expressing a foreign enzyme in malignant cells that converts a nontoxic prodrug into a cytotoxic metabolite. The relative inefficiency of current in situ gene transfer methodology suggests that enzyme/prodrug combinations that produce membrane permeable metabolites will elicit a more favorable therapeutic response. Ideally, the agent produced by the transduced cell “factories” would be cytotoxic toward both proliferating and quiescent cells. We describe a novel GDEPT approach using d-amino acid oxidase from the red yeast Rhodotorula gracilis and d-alanine as a substrate that generates hydrogen peroxide, a reactive metabolite of oxygen that has both these characteristics. We also demonstrate the ability to sensitize tumor cells to this GDEPT protocol by manipulating cellular antioxidant pathways. | en_US |
dc.format.extent | 1367299 bytes | |
dc.format.extent | 2489 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Mary Ann Liebert, Inc., publishers | en_US |
dc.title | Induction of Cytotoxic Oxidative Stress by d-Alanine in Brain Tumor Cells Expressing Rhodotorula gracilis d-Amino Acid Oxidase: A Cancer Gene Therapy Strategy | en_US |
dc.type | Article | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.identifier.pmid | 9472778 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/63220/1/hum.1998.9.2-185.pdf | |
dc.identifier.doi | doi:10.1089/hum.1998.9.2-185 | en_US |
dc.identifier.source | Human Gene Therapy | en_US |
dc.identifier.source | Human Gene Therapy | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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