Nasal Immunization with a Recombinant HIV gp120 and Nanoemulsion Adjuvant Produces Th1 Polarized Responses and Neutralizing Antibodies to Primary HIV Type 1 Isolates
dc.contributor.author | Bielinska, Anna U. | en_US |
dc.contributor.author | Janczak, Katarzyna W. | en_US |
dc.contributor.author | Landers, Jeffrey J. | en_US |
dc.contributor.author | Markovitz, David M. | en_US |
dc.contributor.author | Montefiori, David C. | en_US |
dc.contributor.author | Baker, James R. Jr. | en_US |
dc.date.accessioned | 2009-07-10T19:05:17Z | |
dc.date.available | 2009-07-10T19:05:17Z | |
dc.date.issued | 2008-02-01 | en_US |
dc.identifier.citation | Bielinska, Anna U.; Janczak, Katarzyna W.; Landers, Jeffrey J.; Markovitz, David M.; Montefiori, David C.; Baker, James R. (2008). "Nasal Immunization with a Recombinant HIV gp120 and Nanoemulsion Adjuvant Produces Th1 Polarized Responses and Neutralizing Antibodies to Primary HIV Type 1 Isolates." AIDS Research and Human Retroviruses 24(2): 271-281 <http://hdl.handle.net/2027.42/63251> | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/63251 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=18260780&dopt=citation | en_US |
dc.description.abstract | ABSTRACT Epidemiological and experimental data suggest that both robust neutralizing antibodies and potent cellular responses play important roles in controlling primary HIV-1 infection. In this study we have investigated the induction of systemic and mucosal immune responses to HIV gp120 monomer immunogen administered intranasally in a novel, oil-in-water nanoemulsion (NE) adjuvant. Mice and guinea pigs intranasally immunized by the application of recombinant HIV gp120 antigen mixed in NE demonstrated robust serum anti-gp120 IgG, as well as bronchial, vaginal, and serum anti-gp120 IgA in mice. The serum of these animals demonstrated antibodies that cross-reacted with heterologous serotypes of gp120 and had significant neutralizing activity against two clade-B laboratory strains of HIV (HIVBaL and HIVSF162) and five primary HIV-1 isolates. The analysis of gp120-specific CTL proliferation, INF-γ induction, and prevalence of anti-gp120 IgG2 subclass antibodies indicated that nasal vaccination in NE also induced systemic, Th1-polarized cellular immune responses. This study suggests that NE should be evaluated as a mucosal adjuvant for multivalent HIV vaccines. | en_US |
dc.format.extent | 264822 bytes | |
dc.format.extent | 2489 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Mary Ann Liebert, Inc., publishers | en_US |
dc.title | Nasal Immunization with a Recombinant HIV gp120 and Nanoemulsion Adjuvant Produces Th1 Polarized Responses and Neutralizing Antibodies to Primary HIV Type 1 Isolates | en_US |
dc.type | Article | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.identifier.pmid | 18260780 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/63251/1/aid.2007.0148.pdf | |
dc.identifier.doi | doi:10.1089/aid.2007.0148 | en_US |
dc.identifier.source | AIDS Research and Human Retroviruses | en_US |
dc.identifier.source | AIDS Research and Human Retroviruses | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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